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OALib Journal期刊
ISSN: 2333-9721
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罗伊氏乳杆菌中甘油脱水酶的催化特性及原位再激活
DOI: 10.3724/SP.J.1145.2013.00030, PP. 30-36
Keywords: 罗伊氏乳杆菌,甘油脱水酶,辅酶b12,3-羟基丙醛
Abstract:
对罗伊氏乳杆菌（lactobacillusreuteri）全细胞和粗酶液催化转化甘油和1,2-丙二醇进行比较，分析其底物转化特性；使用有机溶剂制备通透细胞，外源添加辅酶b12和atp，探索l.reuteri胞内甘油脱水酶再激活机制.l.reuteri胞内存在依赖于辅酶b12的甘油脱水酶，1,2-丙二醇不会使甘油脱水酶全酶失活，而甘油会使全酶失活；其最适温度和最适ph分别为37℃和6.2；获得了制备l.reuteri通透细胞的最佳条件；向通透细胞添加辅酶b12和atp均能促进甘油脱水酶催化甘油转化，辅酶b12仅与甘油脱水酶单酶结合形成具催化活性的全酶，而atp能协助失活辅酶脱离全酶和失活辅酶再激活过程.l.reuteri与klebsiellapneumoniae类似，胞内存在甘油脱水酶再激活机制，但来源于二者的甘油脱水酶氨基酸序列同源性较低.来源于l.reuteri的甘油脱水酶是典型的依赖于辅酶b12的甘油脱水酶，尽管其序列同源性与来源于k.pneumoniae、citrobacterfreundii的甘油脱水酶差异较大，但在胞内同样存在甘油脱水酶再激活机制，可利用该机制实现胞内甘油脱水酶原位再激活，进而达到重复利用的目的.
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