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OALib Journal期刊
ISSN: 2333-9721
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甘蔗蔗糖磷酸合成酶spsⅱcdna片段克隆与表达分析
DOI: 10.3724/SP.J.1145.2011.00673, PP. 673-677
Keywords: 蔗糖磷酸合成酶,基因克隆,表达分析,实时荧光定量pcr,甘蔗
Abstract:
蔗糖磷酸合成酶（sucrosephosphatesynthase，sps）是蔗糖合成途径的关键限速酶，在蔗糖积累和碳分配中具有重要作用.在茎组织中spsⅱ表达量占sps转录总量的40%，表明spsⅱcdna的克隆与表达对蔗茎中蔗糖的积累有着重要的影响.通过rt-pcr技术克隆分离spsⅱ基因cdna片段.序列分析表明该cdna片段包含1个3183bp的开放读码框，可编码1060个氨基酸.genbank登录号为eu269038.通过实时荧光定量pcr检测spsⅱ基因在甘蔗糖分积累的不同时期、不同组织部位的相对表达量.结果表明，在糖分积累初期蔗茎中的spsⅱ相对表达量最大，在糖分积累中期蔗叶中的spsⅱ相对表达量达到最高峰；同组织部位中，糖分积累初期spsⅱ相对表达量高于糖分积累中期、后期.图6参11
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