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OALib Journal期刊
ISSN: 2333-9721
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贵州浓香型白酒大曲中霉菌的18srdna系统发育分析
DOI: 10.3724/SP.J.1145.2011.00334, PP. 334-337
Keywords: 丝状真菌,浓香型白酒,大曲,18srdna,系统发育分析,多样性,假埃希氏菌属
Abstract:
从贵州某名酒厂大曲中分离到104株丝状真菌（霉菌），通过形态特征和生理特性检测等传统鉴定方法，初步把形态和生理生化特征一致的霉菌归类为14个大类群；并采用18srdna序列分析，进一步把菌株归类为mj-ⅰ至mj-ⅴ五个类群，随后构建系统发育树.结果表明，样品中的霉菌以曲霉属（aspergillus）为主，占总分离菌数的40.38%；犁头霉属（absidia）也占有相当的比例，达到31.73%；还有16.35%的假埃希氏菌属（pseudallescheria）、6.73%的茎点霉属（phoma）以及4.80%的拟青霉属（paecilomyces）.其中，假埃希氏菌属菌株在相似生态环境中还未见公开报道.mj-ⅰ菌群的代表菌株mj-10与烟曲霉（aspergillusfumigatus）的相似度达到了99%，mj-ⅴ菌群的代表菌株mj-11与伞枝犁头霉（absidiacorymbifera）的相似度达到100%，表明菌株mj-10和菌株mj-11已经鉴定到了种.研究表明，在生态环境有差异的情况下，大曲中微生物的多样性既有相似性，也有所差异，这是贵州浓香型白酒区别于其他地方浓香型白酒的一个重要原因，同时也为贵州浓香型白酒的个性化和品质提升提供了理论依据.图2表1参17
| [1] | 1xiangwl(向文良),zhangwx(张文学),huc(胡承).characterandphylogeneticanalysisofprokaryoticmicroorganisminthestrongaromaticchinesespiritscellar.jsichuanunivenginscied(四川大学学报工程科学版),2005,37(1):39?~40
|
| [2] | 2lizx(李再新),leiyl(雷云玲),liudy(刘达玉).molecularcloningandsequenceanalysisofpartial18srdnaofmoldinkoji.foodsci(食品科学),2009,30(1):211
|
| [3] | 3lizz(李宗珍),zhangsy(张宿义),xudf(许德富),shuily(税梁扬),liugy(刘光烨).distributioncharacteristicsofairbornefungiinsummerinbrewageworkshopsofluzhoulaojiao.chinjapplenvironbiol(应用与环境生物学报),2010,16(1):109~113
|
| [4] | 4张纪忠.微生物分类学.上海:复旦大学出版社,1992
|
| [5] | 8jandajm,abbottsl.bacterialidentificationforpublication:whenisenough?clinmicrobiol,2002,40:1887~1891
|
| [6] | 9yueyy(岳元媛),zhangwx(张文学),liux(刘霞).isolationandidentificationoffacultativeanaerobesinthepitmudofchineseluzhouflavorliquor.microbiology(微生物学通报),2007,34(2):251~255
|
| [7] | 5魏景超.真菌鉴定手册.上海:上课科学技术出版社,1979
|
| [8] | 6wuf(吴飞),chenhy(陈红英),huc(胡承).systematicanalysisof18srdnatotalsequenceofcultivableyeastinluzhouflavorfermentedgrains.liquor-makingsci&technol(酿酒科技),2006,4:24
|
| [9] | 7drancourtm,bolletc,carliozr,etal.16sribosomaldnasequenceanalysisofalargecollectionofenvironmentalandclinicalunidentifiablebacterialisolates.clinmicrobiol,2000,38:3623~3630
|
| [10] | 10liyh(李佑红),wuyy(吴衍庸).acomparativestudyonbacterialgroupsinsichuanaromaticandmellowleavens.microbiology(微生物学通报),1992,19(4):211~214
|
| [11] | 11tangym(唐玉明),yaowc(姚万春),rendq(任道群).analysisofmicrobesinfermentedgrainsofmao-taiflavorliquorduringthefermentationinpits.liquor-makingsci&technol(酿酒科技),2007,162(12):50~53
|
| [12] | 12wanghy(王海燕),zhangxj(张晓君),xuy(徐岩).analysisofthebacterialcommunityinfermentedgrainsofstrongaromastyleandroastedthesamearomastyleliquor.liquor-makingsci&technol(酿酒科技),2008,164(2):86~91
|
| [13] | 13zhaod(赵东),qiaozw(乔宗伟),pengzy(彭志云).investigationthemicroflorainfermentedgrains&theevolutionofitsecologicalfactorsduringthefermentationofluzhouflavorliquor.liquor-makingscience&technol(酿酒科技),2007,157(7):37~39
|
| [14] | 14zhangwx(张文学),qiaozw(乔宗伟).fungaldiversityoffermentedgrainsduringtheproductionofchinesestrongaromaticspirits.jsichuanunivenginscied(四川大学学报工程科学版),2006,38(5):101
|
| [15] | 15lumq(路敏琦),lij(李俊),jiangx(姜昕),lil(李力),shendl(沈德龙),caofm(曹凤明).diversityandphylogenyofrhizobiaisolatedfromthenodulesofbroadbean(viciafabal.)inchina.chinjapplenvironbiol(应用与环境生物学报),2007,13(1):73~77
|
| [16] | 16panf(潘峰),wangp(王平),huzj(胡正嘉),hesj(何绍江),fengxm(冯新梅).phylogenicstudyofrhizobiastrainsisolatedfromricebean(vignaumbellatal.)inhubeiby16s-23srdnarflpand16srrnagenesequencing.chinjapplenvironbiol(应用与环境生物学报),2007,13(1):78~82
|
| [17] | 17fub(付博),wangww(王卫卫),haoy(郝莹),wanglj(王莉娟),tangm(唐明),chenxd(陈兴都).isolationandidenti?cationofhydrogen-oxidizingbacteriainmedicagosativarhizosphere.chinjapplenvironbiol(应用与环境生物学报),2009,15(5):650~654
|
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