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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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响应面法优化paraconiothyriumvariabileghj-4产漆酶发酵条件
DOI: 10.3724/SP.J.1145.2011.00321, PP. 321-325
Keywords: paraconiothyriumvariabileghj-4,漆酶,plackett-burman,响应面,发酵条件优化
Abstract:
为提高子囊菌paraconiothyriumvariabileghj-4产漆酶的能力，采用响应面法对其发酵条件进行优化.首先用plackett-burman法筛选出4个影响较大的重要因素，分别为：接种量（x1）、培养时间（x2）、装样量（x3）和转速（x4）.继而采用最陡爬坡路径逼近最大响应区域，并结合中心组合试验以及响应面分析，建立了以漆酶酶活为响应值的二次回归方程模型，即y=698.72？13.64x1+3.46x2+25.45x3？11.64x4+44.94x1x3+0.31x2x3+23.14x2x4？19.55x3x4？44.65x12？45.07x22？36.52x32？21.60x42.从中获得最优的发酵条件：接种量6.43%（φ），温度28℃，初始ph4，培养时间182h，装液量72.5ml，转速157r/min，种龄96h.采用该发酵条件，供试菌株的漆酶酶活达到710.44u/ml，较优化前漆酶的产量提高了1.73倍，其试验值与预测值基本相符，说明预测模型可靠性高，可应用于漆酶发酵条件的优化.图1表6参20
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