|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
基于do-stat流加培养控制的l-异亮氨酸发酵条件优化
DOI: 10.3724/SP.J.1145.2011.00317, PP. 317-320
Keywords: l-异亮氨酸,溶氧（do）,do-stat,补料分批发酵,乳糖发酵短杆菌
Abstract:
l-异亮氨酸（l-ile）产生菌代谢流的前期研究报道认为，较低的溶氧浓度有利于l-异亮氨酸的积累.为进一步提高l-异亮氨酸的产量，采用do-stat流加培养控制方法，分别研究了溶氧浓度20%、30%、35%和40%下乳糖发酵短杆菌发酵液中葡萄糖浓度的变化以及l-异亮氨酸的合成情况.结果表明，当发酵罐中溶氧浓度控制在20%时，l-异亮氨酸的产量为24.3gl-1，比分批发酵提高了15.7%.其中，生产强度为0.338gl-1h-1，底物转化率为12.2%.图3表1参25
| [1] | 1zhangkx(张克旭).aminoacidfermentationtechnology.beijing,china:chinalightindustrypress(北京:中国轻工业出版社),1992.651~654
|
| [2] | 2zhangwg(张伟国),qianh(钱和).aminoacidproductiontechnologyandapplications.beijing,china:chinalightindustrypress(北京:中国轻工业出版社),1997.252~264
|
| [3] | 4suzukim,satot,kurosea,shiraih,hanabusak.newlow-molecularweightgelatorsbasedonl-valineandl-isoleucinewithvariousterminalgroups.tetrahlett,2005,46(16):2741~2745
|
| [4] | 5sahmh,eggelingl,morbachs,eikmannsb.constructionofl-isoleucineoverproducingstrainsofcorynebacteriumglutamicum.naturwissenschaften,1999,86(1):33~38
|
| [5] | 8ikedas,fujitai,yoshinagaf.screeningofl-isoleucineproducersamongethionineresistantmutantsofl-threonineproducingbacteria.agricbiolchem,1976,40(3):511~516
|
| [6] | 9lij(李进),zhangwg(张伟国).breedingofl-isoleucineproduceranditsoptimalfermentationconditions.jfoodsci&biotechnol(食品与生物技术学报),2006,25(1):54~59
|
| [7] | 10parkjh,leesy.fermentativeproductionofbranchedchainaminoacids:afocusonmetabolicengineering.applmicrobiolbiotechnol,2010,85(3):491~506
|
| [8] | 11changgf(常高峰),chenn(陈宁),pengf(彭飞),xugh(徐国华),zhangkx(张克旭).environmentalfactorsaffectingl-isoleucinefermentation.aminoacids&bioticresour(氨基酸和生物资源),2003,25(4):52~54
|
| [9] | 12liuy(刘勇),zhangck(张长铠),caogy(曹光宇),fangm(房敏).theeffectofbatch-feedingcultureonl-isoleucinefermentation.indmicrobiol(工业微生物),2000,30(2):26~29,33
|
| [10] | 13songwj(宋文军).studyonfed-batchfermentationconditionsofl-isoleucine.jtianjinnormunivnatsciedit(天津师范大学学报自然科学版),2008,28(4):6~8,18
|
| [11] | 14wuzf,dugc,chenj.effectsofdissolvedoxygenconcentrationanddo-statfeedingstrategyoncoq10productionwithrhizobiumradiobacter.worldjmicrobiol&biotechnol,2003,19(9):925~928
|
| [12] | 15yanot,endot,tujit,nishizaway.fed-batchculturewithamodifieddo-statmethod.jferment&bioengin,1991,71(1):35~38
|
| [13] | 21zhangfh(张芙华),huazz(华兆哲),chenj(陈坚),wuj(吴敬).effectoftwo-stagedtemperaturestrategyonproductionofcutinasewithrecombinantbacillussubtilis.chinjapplenvironbiol(应用与环境生物学报),2009,15(5):730~733
|
| [14] | 22qiyz(戚以政),wangsx(汪叔雄).生化反应动力学与反应器.beijing,china:chemicalindustrypress(北京:化学出版社),1999,145~148
|
| [15] | 3suzukim,owas,kimuram,kurosea,shiraih,hanabusak.supramolecularhydrogelsandorganogelsbasedonnovell-valineandl-isoleucineamphiphiles.tetrahlett,2005,46(2):303~306
|
| [16] | 6taylor,matthewf.readytodrinkbeveragecomposition.us,pct/us2004/036572,2006
|
| [17] | 7kisumim.studiesontheisoleucinefermentation.jbiochem,1962,52(6):390~399
|
| [18] | 16liuy(刘勇),chenyl(陈雅丽),zhangck(张长铠),likf(李昆峰).l-异亮氨酸发酵对氧需求的研究.biotechnology(生物技术),1998,8(1):26~28
|
| [19] | 17caoy(曹艳),dingj(丁健),duanzy(段作营),liulm(刘立明),shizp(史仲平).on-lineglucoseconcentrationpredictionandcontrolforimprovingglutamatefermentationperformance.microbiology(微生物学通报),2009,36(10):1619~1624
|
| [20] | 18yanot,kurokawam,nishizaway.optimumsubstratefeedrateinfed-batchculturewiththedo-statmethod.jferment&bioengin,1991,71(5):345~349
|
| [21] | 19yangg(杨革),liuy(刘艳),ligz(李桂芝).effectsofdissolvedoxygenandphonγ-poly(glutamicacid)fermentationbybacilluslicheniformis.chinjapplenvironbiol(应用与环境生物学报),2006,12(6):850~853
|
| [22] | 20ko?rösa,vargazs,molnár-perli.simultaneousanalysisofaminoacidsandaminesastheiro-phthalaldehyde-ethanethiol-9-fluorenylmethylchloroformatederivativesincheesebyhigh-performanceliquidchromatography.jchromatogra,2008,1203(2):146~152
|
| [23] | 23pengzj,fangj,lijh,liul,dugc,chenj,wangxy,ningjf,cailm.combineddissolvedoxygenandphcontrolstrategytoimprovethefermentativeproductionofl-isoleucinebybrevibacteriumlactofermentum.bioprocbiosystengin,2010,33(3):339~345
|
| [24] | 24yangfc,maadw.fed-batchcultureofyeastsaccharomycescerevisiaewithado-statmethodbyafuzzycontroller.bioprocengin,1998,18(2):79~82
|
| [25] | 25akashik,shibaih,hirosey.effectofoxygensupplyonl-lysine,l-threonineandl-isoleucinefermentations.agricbiolchem,1979,43(10):2087~2092
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
