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OALib Journal期刊
ISSN: 2333-9721
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响应面法优化固定化细胞催化α-羟基苯乙酸的合成
DOI: 10.3724/SP.J.1145.2012.00667, PP. 667-671
Keywords: plackett-burman,响应面分析法,固定化细胞,α-羟基苯乙酸,微生物转化,催化合成
Abstract:
采用plackett-burman试验设计法及响应面分析法（rsm），对固定化bacillussp.uli-11菌株生物不对称合成(r)-α-羟基苯乙酸（(r)-hpa）进行优化.首先采用plackett-burman试验设计筛选影响产率的主要因素，再用最陡爬坡方法逼近最大响应区域后用box-behnken试验设计及rsm进行回归分析，得到的最佳条件为温度33℃，增殖时间9.3h，装液量18ml，转速180r/min，ph7.2，接种量2%，种龄16h，在此条件下(r)-hpa的产率为49.61%，比优化前的产率提高了23.7%.图3表6参16
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