|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
dsrna阻断大鼠骨髓源性神经干细胞hes5表达的实验研究
Keywords: 双链rna,hes5,神经干细胞,骨髓源性,rna干涉,免疫印迹
Abstract:
目的观察外源性短双链rna(dsrna)在转录后水平即mrna水平降低基因表达的效率,并对其影响因素进行初步探讨。方法将体外分离培养的大鼠骨髓基质细胞,通过由本实验室配制的特殊培养基诱导成神经干细胞,应用人工合成的dsrna于不同浓度转染神经干细胞,westernbloting检测dsrna是否能阻断转录因子hes5的表达,0.5%锥虫蓝法测定各浓度组中培养细胞活力。结果200~300nmol/l浓度的dsrna能特异有效地阻断hes5的表达,而50~200nmol/l浓度的dsrna有利于干细胞存活。结论dsrna能在神经干细胞内启动rna干扰作用,适宜浓度的dsrna能在特异有效地阻断内源性基因的表达的同时,最大限度地保持细胞活力。
| [1] | savillnj,sherrattja.controlofepidermalstemcellclustersbynotch-mediatedlateralinduction.devbiol[j].2003,258(1):141-53.
|
| [2] | panchisiondm,mckayrd.thecontrolofneuralstemcellsbymorphogenicsignals[j].curropingenetdev,2002,12(4):478-87.
|
| [3] | kageyamar,ohtsukat.thenotch-hespathwayinmammalianneuraldevelopment[j].cellres,1999,9(3):179-88.
|
| [4] | shueydj,mccallusde,giordanot.rnai:gene-silencingintherapeuticintervention[j].drugdiscovtoday,2002,7(20):1040-6.
|
| [5] | shiy.mammalianrnaiforthemasses[j].trendsgenet,2003,19(1):9-12.
|
| [6] | elbashirsm,harborthj,lendeckelw,etal.duplexesof21-nucleotidernasmediaternainterferenceinculturedmammaliancells[j].nature,2001,411(6836):494-8.
|
| [7] | elbashirsm,harborthj,weberk,etal.analysisofgenefunctioninsomaticmammaliancellsusingsmallinterferingrnas[j].methods.2002,26(2):199-213.
|
| [8] | leens,dohjimat,bauerg,etal.expressionofsmallinterferingrnastargetedagainsthiv-lrevtranscriptsinhumancells[j].natbiotechnol,2002,20(5):500-5.
|
| [9] | miyagishim,tairak.u6promoter-drivensirnaswithfoururidine3＇overhangsefficientlysuppresstargetedgeneexpressioninmammaliancells[j].natbiotechnol,2002,20(5):497-500.
|
| [10] | mourrainp,beclinc,elmayant,etal.arabidopsissgs2andsgs3genesarerequiredforposttranscriptionalgenesilencingandnaturalvirusresistance[j].cell,2000,101(5):533-42.
|
| [11] | wu-scharfd,jeongb,zhangc,etal.transgenicandtransposesilencinginchlamydomonasreinhardtiibyadeah-boxrnahelicase[j].science,2000,290(5494):1159-62.
|
| [12] | catalanottoc,azzaling,macinog,etal.genesilencinginwormsandfungi[j].nature,2000,404(6775):245-9.
|
| [13] | catalanottoc,azzaling,macinog,etal.involvementofsmallrnasandroleoftheqdegenesinthegenesilencingpathwayinneurospora[j].genesdev,2002,16(7):790-5.
|
| [14] | bryantj,goodyearrj,richardsongp.sensoryorgandevelopmentintheinnerear:molecularandcellularmechanisms[j].brmedbull,2002,63(1):39-57.
|
| [15] | kumet,jiangh,topczewskajm,etal.themurinewingedhelixtranscriptionfactors,foxclandfoxc2,arebothrequiredforcardiovasculardevelopmentandsomitogenesis[j].genesdev,2001,15(18):2470-82.
|
| [16] | timmonsl,firea.specificinterferencebyingesteddsrna[j].nature,1998,395(6705):854-68.
|
| [17] | maineem.rnaiasatoolforunderstandinggermlinedevelopmentincaenorhabditiselegans:usesandcautions[j]].devbiol,2001,239(2):177-89.
|
| [18] | harborthj,elbashirsm,bechertk,etal.identificationofessentialgenesinculturedmammaliancellsusingsmallinterferingrnas[j].jcellsci,2001,114(24):4557-65.
|
| [19] | timmonsl,courtdl,firea.ingestionofbacteriallyexpresseddsrnascanproducespecificandpotentgeneticinterferenceincaenorhabditiselegans[j].gene,2001,263(1-2):103-12.
|
| [20] | paulcp,goodpd,wineri,etal.effectiveexpressionofsmallinterferingrnainhumancells[j].natbiotechnol,2002,20(5):505-8.
|
| [21] | 王吉兴,姚军.sv40ltag基因永生化软骨细胞体外培养的生物学特性[j].第一军医大学学报,2003,23(12):1338-40.wangjx,yaoj.observationofthebiologicalbehaviorofinvitroculturedimmotalizedchondrocytesinducedbysv40ltaggenetransfection[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(12):1338-40.
|
| [22] | 欧阳平,陈慧萍,赖文岩,等.金钱鱼凋亡相关基因saar对nih-3t3细胞凋亡的影响[j].第一军医大学学报,2003,23(11):1137-8.ouyangp,chenhp,laiwy,etal.effectsofscatophagusargusapoptosis-relatedgeneonapoptosisofnih-3t3cells[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(11):1137-8.
|
| [23] | 罗勇,高建华,赵菲.fas基因转染瘢痕疙瘩成纤维细胞并诱导其凋亡的实验研究[j].第一军医大学学报,2003,23(10):1015-7.luoy,gaojh,zhaof.apoptosisofkeloid-derivedfibroblastsinducedbyfasgenetransfection[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(10):1015-7.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
