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OALib Journal期刊
ISSN: 2333-9721
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超声酶促维生素a棕榈酸酯反应的动力学
, PP. 53-57
Keywords: 超声辐照,维生素a棕榈酸酯,脂肪酶,动力学
Abstract:
？考察超声辐照下影响合成维生素a棕榈酸酯反应的因素(溶剂、底物摩尔比、底物浓度、反应时间、酶量和超声功率)，并优化了反应条件:在10ml的正己烷溶剂中,0.164g维生素a醋酸酯和0.32g棕榈酸,在酶与维生素a醋酸酯的质量比为1∶4的固定化脂肪酶novozym435催化下,超声功率为90w,反应6h,酯化率可达82％。并对其动力学进行了研究,由非线性拟合得到动力学参数：最大反应初速率（rmax）为0.522mmol/（min·g）;维生素a醋酸酯的米氏常数为0.404mmol/l;棕榈酸的米氏常数为0.034mmol/l。
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