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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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植物乳杆菌lactobacilluplantarumy1菌株胆盐水解酶基因(bsh)的克隆及重组表达
, PP. 91-96
Keywords: 植物乳杆菌,胆盐水解酶,原核表达,融合标签技术
Abstract:
经同源蛋白比对分析设计引物,pcr扩增获得植物乳杆菌y1菌株bsh基因(975bp),首先克隆至表达载体pet-28a,转化e.colibl21(de3)菌株,iptg诱导表达,sds-page分析结果显示表达的重组蛋白为包涵体.选择能为大肠杆菌稀有密码子提供额外trna的e.colirosetta(de3)作为宿主菌,仍旧没有改善表达产物的可溶性.但是,选择含if2融合蛋白标签的pls-if2质粒构建表达载体,sds-page分析及westernblot鉴定结果显示表达的融合重组蛋白if2-bs
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