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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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赖氨酸6-氨基转移酶合成条件的优化
, PP. 75-78
Keywords: 赖氨酸6-氨基转移酶,优化,发酵,l-哌可酸
Abstract:
以一株从土壤中分离、筛选到轮枝霉菌(verticilliumsp.)2-14为起始菌株,在摇瓶发酵水平上对其产赖氨酸6-氨基转移酶的条件,包括不同碳源、氮源、磷酸盐、表面活性剂等进行了优化,并对基本无机盐离子进行了正交实验分析.结果表明,该菌株产lat最佳液体发酵培养基为:0？2%l-赖氨酸,3%可溶性淀粉,0？5%kh2po4,0？1%mgso4？7h2o.通过考察菌株生长与产酶进程发现,培养52h时酶活达到峰值1217u？l-1,与优化前相比,酶活力提高一倍.
| [1] | 李振东,樊君,张宗伟.哌啶酸及其衍生物的研究进展[j].化学通报,2006,69:74-80.
|
| [2] | fujiit,naritat,agematuh,eta.lcharacterizationofl-lysine6-aminotransferaseanditsstructiongenefromflavobacter-iumlutescensifo3084[j].jbiochem,2000,128:391-397.
|
| [3] | schmidth,boder,birnbaumda.novelenzyme,l-lysine:pyruvateaminotransferase,catalysesthefirststepoflysinecatabolisminpichiaguilliermondii[j].femsmicrobiologyletter,1988,49:203-206.
|
| [4] | hem.pipecolicacidinmicrobes:biosyntheticroutesandenzymes[j].jindmicrobiolbiotechno,l2006,33(6):401-407.
|
| [5] | patelrn,banerjeea,nandurivb.biocatalyticpreparationofchiralsynthonforavasopeptidaseinhibitor:enzymaticconversionofenzymaticconversionofn2-[n-phenylmethoxy)carbonyl]l-homocysteinyl]-l-lysine(1->1)’-disulfideto[4s-(4i,7i,10aj)]1-octahydro-5-oxo-4-[phenylmethoxy)carbonyl]amino]-7h-pyrido-[2,1-b][1,3]thiazepine-7-carboxylicacidmethylesterbyanovell-lysine-aminotransferase[j].enzymeandmicrobialtechnology,2000,27:376-389.
|
| [6] | fujjit,aritokuy,agematuh,eta.lbiotransformationofl-lysinetol-pipecolicacidcatalyzedbyl-lysine6-aminotransferaseandpyrroline-5-carboxylatereductase[j].bioscibiotechnolbiochem,2002,66:1981-1984.
|
| [7] | martinjf,campoys,naranjol,eta.llysineiscatabolizedto2-aminoadipicacidinpenicilliumchrysogenumbyanx-am-inotransferaseandtosaccharopinebyalysine2-ketoglutaratereductase.characterizationofthex-aminotransferase[j].molgengenomics,2005,274(3):272-282.
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