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OALib Journal期刊
ISSN: 2333-9721
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液态混合菌发酵优化纤维素酶组分
DOI: 10.3969/j.jssn.1000-2006.2011.02.017, PP. 78-82
Keywords: 里氏木霉,黑曲霉,混合培养,纤维素酶,组分优化
Abstract:
在里氏木霉和黑曲霉液态混合条件下培养纤维素酶，分析两个菌种的接种比和延迟黑曲霉的接种时间对产酶的影响，探讨两个菌种发挥协同作用的最佳条件。结果表明：黑曲霉延迟接种48h及里氏木霉与黑曲霉接种质量比1∶1时，所产纤维素酶的滤纸酶活为1？163μmol/(min？ml)；β-葡萄糖苷酶活为0？606μmol/(min？ml)，β-葡萄糖苷酶活与滤纸酶活比值为0？521，比单一里氏木霉产纤维素酶的酶活高，并在后续的酶解效果对比中表现最佳。48h时的酶解得率为65？61%，高于里氏木霉单一培养时所产纤维素酶的得率53？91%和商品纤维素酶的得率49？64%。说明通过混合发酵，纤维素酶的组分得到了优化。
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