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OALib Journal期刊
ISSN: 2333-9721
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柽柳thpr1基因的克隆与表达分析
DOI: 10.3969/j.issn.1000-2006.2013.02.008, PP. 45-49
Keywords: 刚毛柽柳,病程相关蛋白,定量rt-pcr,nacl胁迫,peg胁迫,aba处理
Abstract:
通过对盐胁迫后刚毛柽柳(tamarixhispida)7个转录组分析,鉴定获得病程蛋白相关基因pr1(命名为thpr1)全长cdna序列,该基因全长756bp,编码区长537bp,编码含178个氨基酸的蛋白质,分子质量为20.53ku,理论等电点(pi)为9.75。为了研究该基因对逆境胁迫的应答情况,采用实时定量rt-pcr技术分析了刚毛柽柳在peg、nacl胁迫及外源aba处理后不同组织中基因的表达。结果表明,nacl会诱导thpr1基因在根和叶中的表达,而peg和aba处理则抑制该基因在根和叶中的表达。
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