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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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碧桃花瓣转录组微卫星特征分析
DOI: 10.3969/j.issn.1000-2006.2015.03.007, PP. 34-38
Keywords: 碧桃,微卫星,ssr,转录组,高通量测序
Abstract:
为开发碧桃转录组微卫星信息,利用454高通量测序技术,对其花瓣转录组序列进行ssr位点发掘,结果发现含ssr的序列4705条,共得到5668个ssr,平均每3.49kb出现1个ssr。微卫星序列主要以三碱基重复为主,约占总数的42.66%。笔者共发现516种碱基重复基元,所占比例最高的为(ag/ct)n(18.34%),其次是(aag/ctt)n(12.42%)。微卫星多为重复长度小于20bp的短序列,长度大于20bp的微卫星仅占总数的12.13%。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关(p<0.05),相关系数为-0.246。
| [1] | mrázekj,guox,shaha.simplesequencerepeatsinprokaryoticgenomes[j].proceedingsofthenationalacademyofsciences,2007,104(20):8472-8477.
|
| [2] | tóthg,gáspáriz,jurkaj.microsatellitesindifferenteukaryoticgenomes:surveyandanalysis[j].genomeresearch,2000,10(7):967-981.
|
| [3] | 杨华,陈琪,韦朝领,等.茶树转录组中ssr位点的信息分析[j].安徽农业大学学报,2011,38(6):882-886.yangh,chenq,weizl,etal.analysisonssrinformationincamelliasinensistranscriptome[j].journalofanhuiagriculturaluniversity,2011,38(6):882-886.
|
| [4] | 李小白,向林,罗洁,等.转录组测序(rna-seq)策略及其数据在分子标记开发上的应用[j].中国细胞生物学报,2013,35(5):1-8.lixb,xiangl,luoj,etal.thestrategyofrna-seq,applicationanddevelopmentofmolecularmarkerderivedfromrna-seq[j].chinesejournalofcellbiology,2013,35(5):1-8.
|
| [5] | cheny,maoy,liuh,etal.transcriptomeanalysisofdifferentiallyexpressedgenesrelevanttovariegationinpeachflowers[j].plosone,2014,9(3):e90842.
|
| [6] | verdei,abbottag,scalabrins,etal.thehigh-qualitydraftgenomeofpeach(prunuspersica)identifiesuniquepatternsofgeneticdiversity,domesticationandgenomeevolution[j].naturegenetics,2013,45(5):487-494.
|
| [7] | biete,sunj,dutreixm.conservedsequencepreferenceindnabindingamongrecombinationproteins:aneffectofssdnasecondarystructure[j].nucleicacidsresearch,1999,27(2),596-600.
|
| [8] | 何平.真核生物中的微卫星及其应用[j].遗传,1998,20(4):42-47.hep.abundance,polymorphismandapplicationsofmicrosatelliteineukaryote[j].hereditas,1998,20(4):42-47.
|
| [9] | 杨纪青,杨硕,杨莉,等.tomatoleafcurlbangladesh病毒完整基因组上微卫星分布[j].中国农学通报,2010,26(24):20-27.yangjq,yangs,yangl,etal.themicrosatellitedistributioninthecompletegenomesofthetomatoleafcurlbangladeshvirus[j].chineseagriculturalsciencebulletin,2010,26(24):20-27.
|
| [10] | selkoeka,toonenrj.microsatellitesforecologists:apracticalguidetousingandevaluatingmicrosatellitemarkers[j].ecologyletters,2006,9(5):615-629.
|
| [11] | beheregaraylb,ciofic,geistd,etal.genesrecordaprehistoricvolcanoeruptioninthegalapagos[j].science,2003,302(5642):75.
|
| [12] | 袁阳阳,王青锋,陈进明.基于转录组测序信息的水生植物？菜ssr标记开发[j].植物科学学报,2013,31(5):485-492.yuanyy,wangqf,chenjm.developmentofssrmarkersinaquaticplantnymphoidespeltata(menyanthaceae)basedoninformationfromtranscriptomesequencing[j].journalofplantsciences,2013,31(5):485-492.
|
| [13] | 孔凡明,王小龙,陈赢男,等.松属编码区微卫星特征和相应基因功能分析[j].南京林业大学学报:自然科学版,2014,38(2):47-51.kongfm,wangxl,chenyn,etal.characterizationofmicrosatellitesincodinggenesandfunctionalanalysisofthesegenesinpines[j].journalofnanjingforestryuniversity:naturalsciencesedition,2014,38(2):47-51.
|
| [14] | 张文娥,潘学军,杨仕国,等.碧桃组织培养中褐化及其抑制研究[j].贵州农业科学,2009,37(1):11-12.zhangwe,panxj,yangsg,etal.inhibitioneffectsofdifferentsterilizedmethods,inoculationtimesandantioxidantsonbrowningintissuecultureofprunuspersicavar.duplex[j].guizhouagriculturalscience,2009,37(1):11-12.
|
| [15] | 李雄伟,贾惠娟,高中山.桃基因组学及全基因组关联分析研究进展[j].遗传,2013,35(10):1167-1178.lixw,jiahj,gaozs.peachgenomicsandgenome-wideassociationstudy:areview[j].hereditas,2013,35(10):1167-1178.
|
| [16] | 曹珂,王力荣,朱更瑞,等.桃遗传图谱的构建及两个花性状的分子标记[j].园艺学报,2009,36(2):179-186.caok,wanglr,zhugr,etal.geneticlinkagemapsconstructingandmarkerslinkedwithpistildevelopmentanddoublepetalgeneinpeach[j].actahorticulturaesinica,2009,36(2):179-186.
|
| [17] | weberjl.informativenessofhuman(dc-da)n？(dg-dt)npolymorphisms[j].genomics,1990,7(4):524-530.
|
| [18] | wangl,zhaos,guc,etal.deeprna-sequncoversthepeachtranscriptomelandscape[j].plantmolecularbiology,2013,83(4-5):365-377.
|
| [19] | cavagnaropf,senalikda,yangl,etal.genome-widecharacterizationofsimplesequencerepeatsincucumber(cucumissativusl.)[j].bmcgenomics,2010,11:569.
|
| [20] | 陈军方,任正隆,高丽锋,等.从小麦est序列中开发新的ssr引物[j].作物学报,2005,31(2):154-158.chenjf,renzl,gaolf,etal.developingnewssrmarkerfromestofwheat[j].actaagronomicasinica,2005,31(2):154-158.
|
| [21] | 江东,钟广炎,洪棋斌.柑橘est-ssr分子标记分析[j].遗传学报,2006,33(4):345-353.jiangd,zhonggy,hongqb.analysisofmicrosatellitesincitrusunigenes[j].actageneticasinica,2006,33(4):345-353.
|
| [22] | 杨素丽,明军,刘春,等.基于est信息的百合ssr标记的建立[j].园艺学报,2008,35(7):1069-1074.yangsl,mingj,liuc,etal.dataminingforsimplesequencerepeatsmarkerdevelopmentinexpressedsequencetagsfromliliuml.[j].actahorticulturaesinica,2008,35(7):1069-1074.
|
| [23] | 王丽鸳,韦康,张成才,等.茶树花转录组微卫星分布特征[j].作物学报,2014,40(1):80-85.wangly,weik,zhangcc,etal.characterizationofmicrosatellitesintea(camelliasinensis)floraltranscriptome[j].actaagronomicasinica,2014,40(1):80-85.
|
| [24] | 王艳敏,魏志刚,杨传平.白桦est-ssr信息分析与标记的开发[j].林业科学,2008,44(2):78-84wangym,weizg,yangcp.dataminingforssrsinestsandest-ssrmarkerdevelopmentinbetulaplatyphylla[j].scientiasilvaesinicae,2008,44(2):78-84.
|
| [25] | 朱艳,郝艳宾,王克建,等.核桃est-ssr信息分析与标记的初步建立[j].果树学报,2009,26(3):394-398.zhuy,haoyb,wangkj,etal.analysisofssrsinformationanddevelopmentofssrmarkersfromwalnutests[j].journaloffruitscience,2009,26(3):394-398.
|
| [26] | 姜春芽,徐小彪,廖娇,等.猕猴桃est序列的ssr信息分析[j].中国农学通报,2009,25(13):37-39.jiangcy,xuxb,liaoj,etal.analysisofssrinformationinestresourcesofkiwifruit(actinidiassp.)[j].chineseagriculturalsciencebulletin,2009,25(13):37-39.
|
| [27] | 张新叶,宋丛文,张亚东,等.杨树est-ssr标记的开发[j].林业科学,2009,45(9):53-59.zhangxy,songcw,zhangyd,etal.developmentofest-ssrinpopulusdeltodesandp.euramericana[j].scientiasilvaesinicae,2009,45(9):53-59.
|
| [28] | aggarwalrk,hendreps,varshneyrk,etal.identification,characterizationandutilizationofest-derivedgenicmicrosatellitemarkersforgenomeanalysesofcoffeeandrelatedspecies[j].theoreticalandappliedgenetics,2007,114(2):359-372.
|
| [29] | blancaj,ca?izaresj,roigc,etal.transcriptomecharacterizationandhighthroughputssrsandsnpsdiscoveryincucurbitapepo(cucurbitaceae)[j].bmcgenomics,2011,12(1):104.
|
| [30] | 李炎林,杨星星,张家银,等.南方红豆杉转录组ssr挖掘及分子标记的研究[j].园艺学报,2014,41(4):735-745.liyl,yangxx,zhangjy,etal.studiesonssrmolecularmarkersbasedontranscriptomeoftaxuschinensisvar.mairei[j].actahorticulturaesinica,2014,41(4):735-745.
|
| [31] | kantetyrv,larotam,matthewsde,etal,dataminingforsimplesequencerepeatsinexpressedsequencetagsfrombarley,maize,rice,sorghumandwheat[j].plantmolecularbiology,2002,48(5-6):501-510.
|
| [32] | schorderetdf,gartlersm.analysisofcpgsuppressioninmethylatedandnonmethylatedspecies[j].pnas,1992,89(3):957-961.
|
| [33] | gaol,tangj,lih,etal.analysisofmicrosatellitesinmajorcropsassessedbycomputationalandexperimentalapproaches[j].molecularbreeding,2003,12(3):245-261.
|
| [34] | 李小白,张明龙,崔海瑞.油菜est资源的ssr信息分析[j].中国油料作物学报,2007,29(1):20-25.lixb,zhangml,cuihr.analysisofssrinformationinestresourceofoilseedrape[j].chinesejournalofoilcropsciences,2007,29(1):20-25.
|
| [35] | sharopovan.plantsimplesequencerepeats:distribution,variation,andeffectsongeneexpression[j].genome,2008,51(2):79-90.
|
| [36] | temnykhs,declerckg,lukashovaa,etal.computationalandexperimentalanalysisofmicrosatellitesinrice(oryzasatival.):frequency,lengthvariation,transposonassociations,andgeneticmarkerpotential[j].genomeresearch,2001,11(8):1441-1452.
|
| [37] | samadis,artiguebiellee,estoupa,etal.densityandvariabilityofdinucleotidemicrosatellitesintheparthenogeneticpolyploidsnailmelanoidestuberculata[j].molecularecology,1998,7(9):1233-1236.
|
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