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OALib Journal期刊
ISSN: 2333-9721
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培养基组成对里氏木霉合成β-甘露聚糖酶的影响
DOI: 10.3969/j.issn.1000-2006.2013.01.018, PP. 101-104
Keywords: β-甘露聚糖酶,里氏木霉,培养基优化
Abstract:
通过改变产酶培养基中的营养成分与比例,采用酶活力测定方法,分析培养基中的碳源种类和浓度、氮源组成、碳氮比(mc:mn)等主要因素对里氏木霉合成β-甘露聚糖酶的影响。结果表明,20g/l微晶纤维素为碳源、含氮素质量比为1:1的硫酸铵和尿素为氮源、mc:mn=4的培养基组成,是里氏木霉合成β-甘露聚糖酶的最佳条件。在此条件下β-甘露聚糖酶酶活力在发酵96h时达到最大,β-甘露聚糖酶酶活力和β-甘露糖苷酶酶活力分别为4.48、0.04μmol/(min？ml)。
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