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- 2015
NDV、KLT及其联用时小鼠H22肝癌瘤组织的病理变化与TNF-α表达的研究
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Abstract:
【目的】研究新城疫病毒(New Castle Disease Virus，NDV)、康莱特注射液 (Kanglaite Injection，KLT)及NDV+KLT联用治疗肝癌肿瘤过程中，癌组织的病理变化以及肿瘤坏死因子-α(Tumor necrosis factor-alpha，TNF-α)在肿瘤组织中的表达情况，探究TNF-α在抗肿瘤过程中的作用，为应用生物素治疗肿瘤提供一定的理论依据。【方法】建立小鼠H22肝癌肿瘤模型，将96只荷瘤小鼠随机均分成生理盐水对照组（0.8 mL/只）、NDV治疗组（0.8 mL/只）、KLT治疗组（0.5 mL/只）及NDV+KLT联合治疗组共4组，分别在治疗后的4，8，12和16 d取小鼠肿瘤组织制作2套石蜡切片，一套进行HE染色检测病理变化；另一套进行免疫组织化学SP观察TNF-α的表达情况。【结果】HE染色发现，与对照组相比， NDV组和KLT组都有明显的肿瘤生长抑制及肿瘤细胞坏死现象，特别是NDV+KLT联合治疗组的治疗效果显著；免疫组化研究结果表明，不同的治疗组TNF-α分布差异显著，其中NDV+KLT联合治疗组的阳性反应最强，其次是KLT治疗组。【结论】NDV和KLT的抗肿瘤作用与TNF-α细胞因子表达有一定的相关性，其中NDV+KLT联合治疗的抗肿瘤效果最好。
【Objective】This study used New Castle Disease Virus (NDV),Kanglaite Injection (KLT) and their combination to treat liver cancer.The pathological changes of tumor tissue and expression of tumor necrosis factor-alpha (TNF-α) were analyzed to understand the function of TNF-α in cancer treatment.【Method】Mice H22 tumor model was established with 96 tumor-bearing mice randomly divided into four groups,NS control group (0.8 mL),NDV treatment group (0.8 mL),KLT treatment group (0.5 mL) and NDV+KLT treatment group.Tumor tissue was obtained from every group in the 4,8,12 and 16 d respectively.Paraffin sections were made to observe pathological changes using hematoxylin-eosin (HE) staining and expression of TNF-α using immunohistochemistry SP method.【Result】HE staining showed that,compared with NS control group,tumor growth inhibition and tumor cells necrosis appeared,especially NDV+KLT treatment group had significant treatment effect.Compared with NS control group,pathological changes of tumor tissue and distribution of TNF-α were significantly different in all treatment groups,especially in NDV+KLT group.NDV+KLT group also had the strongest positive reaction,followed by KLT treatment group.【Conclusion】Anti-tumor effect of NDV and KLT was related to expression of TNF-α and combination of NDV and KLT had the best effect
