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食品科学  2002 

primaryscreeningof10-hydroxy-2-decenoicacidproductivestrains

Keywords: hydroxy-2-decenoicacid(10-hda)biosynthesisstrainscreening

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Abstract:

?inthispaper,elevenstrains,whichwerescreenedstrictlyfromrawroyaljelly,soilandhoneycombetc.bymeansofdilutionplateandspreadplatemethods,wereculturedat28℃for60hwithshaking.todeterminewhethertheycouldyield10-hydroxy-2-decenoicacidduringfermentation,gaschromatographyandgaschromatography-massspectrometrymethodswereused.theresultsshowedthatthestrainsbh002andbh004,werebothidentifiedascrytococcaceae,wherebh002wasprimarilyclassifiedintocandidaforpossessingtheabilities.the10-hdaproductivityofcandidabh002andthatofbh004were0.327%and0.2648%respectively.

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