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OALib Journal期刊
ISSN: 2333-9721
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雷公藤内酯醇对人lncap细胞雄激素受体表达的调控及其机制
, PP. 1246-1251
Keywords: 雷公藤内酯醇,前列腺癌,雄激素受体,pi3k/akt/nf-κb通路
Abstract:
本文研究了雷公藤内酯醇(triptolide,tp)对人前列腺癌lncap细胞内雄激素受体(androgenreceptor,ar)表达影响的作用机制。采用实时定量pcr和westernblot方法检测tp处理后lncap细胞中ar的mrna和蛋白表达的变化,以及使用tp和nf-κb抑制剂共处理后lncap细胞ar蛋白表达的变化;采用rf克隆法构建系列pgl3-ar启动子报告基因载体并转染至lncap细胞,检测tp对lncap细胞ar转录的调节作用;采用westernblot检测可能起调节作用的上游蛋白。结果表明,tp处理lncap细胞48h后,lncap细胞ar的mrna和蛋白的表达随着药物浓度增加而下降,同时ar靶基因part1及前列腺特异性抗原(prostatespecificantigen,psa)的表达也受到抑制;成功构建系列pgl3-ar启动子报告基因载体,测序结果表明,插入的目的序列正确且无碱基突变,转染lncap细胞后双荧光素酶报告基因检测系统证实其具有启动子活性;启动子报告基因实验结果表明,tp在转录水平下调ar的表达;与ar启动子活性相关的pi3k/akt/nf-κb通路蛋白表达下调。说明tp可能通过pi3k/akt/nf-κb通路下调人前列腺癌lncap细胞ar的转录活性。
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