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早胜牛类群A-FABP基因SNPs及其与胴体品质和肉质性状的相关性分析

, PP. 96-101

Keywords: 早胜牛,A-FABP基因,SNPs,胴体品质,肉质性状

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Abstract:

研究早胜牛类群A-FABP基因多态性及其与胴体品质和肉质性状的相关性。采用PCR-SSCP方法对5个早胜牛类群(庆阳类群、平凉类群、南德温与庆阳类群杂种、西门塔尔与平凉类群杂种、秦川牛与平凉类群杂种)的A-FABP基因进行多态性分析,分析基因型与胴体品质和肉质性状的相关性。结果显示,A-FABP基因第三外显子区存在c.408G>C的突变,并检测到3种基因型GG、GC和CC。胴体性状相关分析表明,GG基因型的胴体重显著低于CC基因型(P<0.05);GG基因型屠宰率极显著低于GC基因型(P<0.01),显著低于CC基因型(P<0.05);GG基因型净肉率显著低于GC基因型(P<0.05);GG基因型的眼肌面积显著低于GC和CC基因型(P<0.05)。肉质性状相关分析表明,GG基因型失水率显著高于CC基因型个体(P<0.05);GG基因型剪切力极显著高于GC和CC基因型(P<0.01);GG基因型蒸煮损失和pH均显著高于GC基因型(P<0.05),极显著高于CC基因型(P<0.01)。A-FABP基因突变位点可作为胴体性状和肉质性状遗传标记。

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