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一株筛选自高寒草地木聚糖酶产生真菌的鉴定及酶学特性研究

, PP. 162-178

Keywords: 高寒草地,木聚糖酶,ITS-rDNA,pH稳定性,生物学特性

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Abstract:

为研究一株分离、筛选自高寒草地土壤中的真菌,采用ITS-rDNA基因序列分析法、液体摇瓶发酵法和传统微生物培养法,对供试菌株的分类学地位进行了确定,并对该菌株产木聚糖酶的酶学性质及其主要生物学特性进行了测定。结果表明,经ITS-rDNA基因序列分析法鉴定,供试菌株初步确定为Saccharicolabicolor。供试菌株S.bicolor可分泌木聚糖酶,且在pH为3.0-9.5时,酶活力相对稳定,表明S.bicolor所分泌的木聚糖酶具有一定的耐受碱性条件的能力。供试菌株S.bicolor在温度为35℃时生长较快,对5种碳源利用情况的先后顺序为:麦芽糖>淀粉>蔗糖>糊精>葡萄糖,对5种氮源利用情况的先后顺序为:蛋白质>磷酸铵>硫酸铵>硝酸钠>尿素。

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