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转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.09.011, PP. 84-90

Keywords: 转基因水稻,肉鸡,基因及蛋白残留,体外消化

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Abstract:

旨在研究转基因水稻对饲喂肉鸡的影响,以含有转cry1Ab/Ac基因水稻的饲料饲喂肉鸡,分析了肉鸡空肠、回肠、盲肠、直肠中cry1Ab/Ac基因和蛋白残留情况,并用体外实验验证了外源基因的降解情况。结果表明,饲喂转cry1Ab/Ac基因水稻21d和42d的肉鸡肠道4个部位中没有检测到cry1Ab/Ac基因残留,体外消化外源基因的实验发现外源基因在肉鸡肠道中约4h后开始逐步降解。对上述4个部位内容物进行ELISA和Westernblot分析,均未检测到Cry1Ab/Ac蛋白残留。综合分析表明,转基因水稻中的外源基因和蛋白在肉鸡肠道中易被消化和降解。

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