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重组β-环糊精葡萄糖基转移酶生产β-环糊精的工艺条件优化

, PP. 175-181

Keywords: 环糊精葡萄糖基转移酶,β-环糊精,酶转化,重组表达,淀粉

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Abstract:

将来自于Bacilluscirculans251的β-CGTase编码基因克隆到表达载体pET-20b(+),转化EscherichiacoliBL21(DE3)。经酶活检测培养基上清中的β-CGTase酶活为20U/mL。对酶转化淀粉生成β-环糊精的反应条件进行了优化,结果表明,当底物马铃薯淀粉浓度15%,反应初始pH5.5,温度30℃,加酶量10U/g干淀粉,环己烷浓度2.5%-5%(V/V),转化周期24h,β-环糊精转化率达到最高值75.3%,是国内外报道的酶法生产β-环糊精的最高水平。

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