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基于高通量测序的辽东栎转录组学研究

, PP. 119-124

Keywords: 辽东栎,IlluminaSolexa,转录组,淀粉,SSR

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Abstract:

应用IlluminaSolexaHiseq2000高通量测序技术对辽东栎的芽、花、叶及果实的混合样品进行转录组测序,结果共获得3.8Gb的有效数据。应用Trinity软件对有效序列从头拼接去重复后,共获得95800条unigene,总长度为73.57Mb,最大长度、平均长度和N50分别为11284bp、768bp和1373bp。利用Blastx与公共数据库Nr和Swiss-Prot的同源性比较(E值<1×10-5)发现,38163条unigene未发现与公共数据库中的序列具有同源性。通过KEGG数据库中参与淀粉合成与代谢的pathway分析,共发掘出67条参与淀粉合成的unigene,编码9个关键酶。此外,在13380条unigene中共搜索到15901个SSR位点,其中二核苷酸和三核苷酸的重复类型占所有SSR位点的98.16%。

References

[1]  Balat M, Balat H. Recent trends in global production and utilization of bio-ethanol fuel [J]. Applied Energy, 2009, 86:2273-2282.
[2]  Hahn-H?gerdal B, Galbe M, Gorwa-Grauslund MF, et al. Bio-ethanol-the fuel of tomorrow from the residues of today[J]. Trends in Biotechnology, 2006, 24(12):549-556.
[3]  谢光辉, 郭兴强, 王鑫, 等.能源作物资源现状与发展前景[J].资源科学, 2007, 29(5):74-80.
[4]  吴创之, 周肇秋, 阴秀丽, 等.我国生物质能源发展现状与思考[J].农业机械学报, 2009, 40(1):91-99.
[5]  李碧芳.发展生物质能源对能源安全和粮食安全的影响[J].生态经济, 2010, 3:41-42.
[6]  方炎明, 张聪颖, 虞木奎, 等.栎树繁殖生物技术进展[J].生物技术通报, 2011(4):60-65.
[7]  陈婧, 马履一, 段劼, 等.中国林业生物质能源发展现状[J].林业实用技术, 2012, 11:16-19.
[8]  田玉峰, 李安平, 谢碧霞, 等.橡实淀粉生物乙醇化橡实品种和菌种的筛选[J].食品科学, 2011, 32(7):207-210.
[9]  李安平, 田玉峰, 谢碧霞, 等.橡实淀粉生料酒精发酵与传统酒精发酵的能耗和成分组成比较[J].江西农业大学学报, 2012, 34(5):1032-1038.
[10]  Soni PL, Sharma H, Dun D, et al. Physicochemical properties of Quercus leucotrichophora(Oak)starch [J]. Starch/St?rke, 1993, 45(4):127-130.
[11]  Stevenson DG, Jane JL, Inglett GE. Physicochemical properties of pin oak(Quercus palustris Muenchh.)acorn starch [J]. Starch/St?rke, 2006, 58(11):553-560.
[12]  Derory J, Léger P, Garcia V, et al. Transcriptome analysis of bud burst in sessile oak(Quercus petraea)[J]. New Phytologist, 2006, 170(4):723-738.
[13]  Lesur I, Durand J, Sebastiani F, et al. A sample view of the pedunculate oak(Quercus robur)genome from the sequencing of hypomethylated and random genomic libraries [J]. Tree Genetics & Genomes, 2011, 7(6):1277-1285.
[14]  Ueno S, Le Provost G, Léger V, et al. Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species:oak [J]. BMC Genomics, 2010, 11:650.
[15]  刘红亮, 郑丽明, 刘青青, 等.非模式生物转录组研究[J].遗传, 2013, 35(8):955-970.
[16]  罗伟祥, 郝怀晓, 薛安平.橡树资源-优质林木生物质能源发展战略研究[J].生物质化学工程, 2006, 40(B12):147-152.
[17]  Huang LL, Yang X, Sun P, et al. The first Illumina-based de novo transcriptome sequencing and analysis of safflower flowers [J]. PloS One, 2012, 7(6):e38653.
[18]  Haas BJ, Papanicolaou A, Yassour M, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis [J]. Nature Protocols, 2013, 8(8):1494-1512.
[19]  Wang R, Xu S, et al. De novo sequence assembly and characteriza-tion of Lycoris aurea transcriptome using GS FLX Titanium platform of 454 pyrosequencing [J]. PloS One, 2013, 8(4):e60449.
[20]  Tao X, Fang Y, Xiao Y, et al. Comparative transcriptome analysis to investigate the high starch accumulation of duckweed(Landoltia punctata)under nutrient starvation [J]. Biotechnology for Biofuels, 2013, 6(1):72.
[21]  Kaminski KP, Petersen AH, S?nderk?r M, et al. Transcriptome analysis suggests that starch synthesis may proceed via multiple metabolic routes in high yielding potato cultivars [J]. PloS One, 2012, 7(12):e51248.
[22]  Shu S, Chen B, et al. De novo sequencing and transcriptome analysis of Wolfiporia cocos to reveal genes related to biosynthesis of triterpenoids [J]. PloS One, 2013, 8(8):e71350.
[23]  Zhou Y, Gao F, Liu R, et al. De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus [J]. BMC Genomics, 2012, 13:266.
[24]  Kole, Chittaranjan. Fagaceae Trees. //In genome mapping and molecular breeding in plants[M] . Springer, 2007, 7:161-184.
[25]  Li SW, Yang H, Liu YF, et al. Transcriptome and gene expression analysis of the rice leaf folder, Cnaphalocrosis medinalis [J]. PloS One, 2012, 7(11):e47401.
[26]  O’Malley DM, McKeand SE. Marker assisted selection for breeding value in forest trees [J]. Forest Genetics, 1994, 1:207-218.
[27]  Neale DB, Kremer A. Forest tree genomics:growing resources and applications [J]. Nature Reviews Genetics, 2011, 12:111-122.

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