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毕赤酵母表面展示棘孢曲霉β-葡萄糖苷酶催化合成APG的工艺条件优化

, PP. 205-210

Keywords: 细胞表面展示,β-葡萄糖苷酶,全细胞催化剂,烷基糖苷

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Abstract:

使用毕赤酵母细胞表面展示棘孢曲霉的β-葡萄糖苷酶作为全细胞催化剂,以葡萄糖为底物,在水-醇双相体系中逆水解合成C6-C10烷基糖苷。讨论了含水量、葡萄糖添加量、全细胞催化剂添加量、乙酸-乙酸钠缓冲液pH值和温度等主要因素对反应的影响,并与商品酶苦杏仁β-葡萄糖苷酶、Novozym188进行比较。结果表明,Novozym188不适合逆水解合成APG,苦杏仁β-葡萄糖苷酶反应所需时间短,但最终转化率不如全细胞催化剂。在5mL反应体系中,合成HG的最优条件:葡萄糖0.1g,全细胞催化剂0.05g,10%pH3.0buffer;合成OG的最优条件:葡萄糖0.2g,全细胞催化剂0.05g,15%pH3.0buffer;合成DG的最优条件:葡萄糖0.2g,全细胞催化剂0.2g,20%pH3.0buffer;放置55℃200r/min恒温振荡器中,反应时间为72h,HG和DG的最大产率分别为11.69%和3.58%,而OG的产率则在96h到达最大值6.34%。

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