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不同水平精氨酸-甘氨酸-谷氨酰胺对断奶仔猪空肠体外酶活及细胞增殖与凋亡的影响

DOI: 10.3969/j.issn.1006-267x.2011.09.004

Keywords: 精氨酸-甘氨酸-谷氨酰胺,断奶仔猪,空肠,细胞增殖,细胞凋亡

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Abstract:

本试验旨在研究精氨酸-甘氨酸-谷氨酰胺(Arg-Gly-Gln)对断奶仔猪空肠体外酶活及细胞增殖与凋亡的影响。试验采用单因子设计,设6个处理,培养液分别添加Arg-Gly-Gln0、0.28、0.56、1.11、2.23、4.45mmol/L,每个处理8个重复,每个重复1个空肠组织块。检测培养液中乳酸脱氢酶(LDH)活力、肠组织中谷氨酰胺酶和二胺氧化酶(DAO)活力以及5-溴脱氧尿嘧啶核苷(BrDU)、半胱氨酸的天冬氨酸特异性蛋白水解酶-3(Caspase-3)阳性细胞百分比的变化。结果表明:1)与无添加的培养液相比,除添加0.56、2.23mmol/LArg-Gly-Gln在6h时以及添加1.11mmol/LArg-Gly-Gln在24h时无显著差异(P>0.05)外,其余各时间点、各添加Arg-Gly-Gln的培养液中LDH活力均显著(P<0.05)或极显著(P<0.01)降低;2)与无添加的培养液相比,培养液添加1.11、2.23、4.45mmol/LArg-Gly-Gln空肠组织谷氨酰胺酶活力分别提高了27.69%、46.15%、83.08%(P<0.01),培养液添加2.23、4.45mmol/LArg-Gly-Gln空肠组织DAO活力分别提高了66.34%和110.89%(P<0.01);3)随着培养液Arg-Gly-Gln水平的提高,BrdU阳性细胞百分比增加,Caspase-3阳性细胞百分比降低。结果提示:Arg-Gly-Gln能够缓解细胞损伤并降低细胞膜通透性,提高空肠组织谷氨酰胺酶和DAO的活力,增强肠细胞对Gln的利用;Arg-Gly-Gln能够促进断奶仔猪离体空肠细胞增殖,抑制由Caspase-3介导的细胞凋亡,从而提高肠道黏膜的屏障功能。

References

[1]  LI Y S, LI J S, JIANG J W, et al. Glycyl-glutamine-supplemented long-term total parenteral nutrition selectively improves structure and function in heterotopic small-bowel autotransplantation in the pig[J]. Transplant International, 2003, 16(12):866-871.
[2]  SCHEPPACH W, LOGES C, BARTRAM P, et al. Effect of free glutamine and alanyl-glutamine dipeptide on mucosal proliferation of the human ileum and colon[J]. Gastroenterology, 1994, 107(2):429-434.
[3]  HAYNES T E, LI P, LI X, et al. L-glutamine or L-alanyl-L-glutamine prevents oxidant or endotoxin-induced death of neonatal entrecotes[J].Amino Acids, 2009, 37(1):131-142.
[4]  BRANDSCH M, KNTTER I, LEIBACH F H. The intestinal H+/peptide symporter PEPT1: structure-affinity relationships[J]. European Journal of Pharmaceutical Sciences, 2004, 21(1):53-60.
[5]  HUANG L, JIA G, WU C M, et al. The absorption and transportation of glutamine containing small peptides Arg-Gly-Gln and Gly-Gln in jejunum of weaned piglets[J]. Agricultural Science and Technology, 2010, 4(5):17-23.
[6]  邱曙东,宋天保.组织化学与免疫组织化学[M].北京:科学技术出版社,2008.
[7]  洪庆涛,宋岳涛,唐一鹏,等.细胞培养液乳酸脱氢酶漏出率的比色测定及其应用[J].细胞生物学杂志,2004,26(1):89-92.
[8]  LEGRAND C, BOUR J M, JACOB C, et al. Lactate dehydrogenase (LDH) activity of the number of dead cells in the medium of cultured eukaryotic cells as marker[J]. Journal of Biotechnology, 1992, 25(3):231-243.
[9]  KLIMBERG V S, SOUBA W W, SALLOUM R M, et al. Intestinal glutamine metabolism after massive small bowel resection[J]. American Journal of Surgery, 1990, 159(1):27-32.
[10]  PINKUS L M, WINDMUELLER H G. Phosphate dependent glutaminase in the small intestine[J]. Archives of Biochemistry and Biophysics, 1977, 182(2):506-517.
[11]  谢建新,顾岩,刘银坤,等.联合应用GH和Gln对短肠大鼠小肠黏膜上皮细胞谷氨酰胺酶和鸟氨酸脱羧酶活性的影响[J].解剖学杂志,2002,25(4):336-339.
[12]  SATOH J, TSUJIKAWA T, FUJIYAMA Y, et al. Enteral alanyl-glutamine supplement promotes intestinal adaptation in rats[J]. International Journal of Molecular Medicine, 2003, 12(4):615-620.
[13]  HAQUE S M, CHEN K, USUI N, et al. Alanyl-glutamine dipeptide-supplemented parenteral nutrition improves intestinal metabolism and prevents increased permeability in rats[J]. Annals of Surgery, 1996, 223(3):334-341.
[14]  TAZUKE Y, WASA M, SHIMIZU Y, et al. Alanyl-glutamine-supplemented parenteral nutrition prevents intestinal ischemia-reperfusion injury in rats[J]. Journal of Parenteral and Enteral Nutrition, 2003, 27(2):110-115.
[15]  BUFFONI F. Histaminase and related amine oxidases[J]. Pharmacological Reviews, 1966, 18(4):1163-1199.
[16]  THOMPSON J S, VAUGHAN W P, FORST C F, et al. The effect of the route of nutrient delivery on gut structure and diamine oxidase levels[J]. Journal of Parenteral and Enteral Nutrition, 1987, 11(1):28-32.
[17]  HESTERBERG R, SATTLER J, LORENZ W, et al. Histamine content, diamine oxidase activity and histamine methyltransferase activity in human tissues: fact or fictions[J]. Agents Actions, 1984, 14(3/4):325-334.
[18]  FOGEL W A. Mucosal mono- and polyamine oxidase activities in digestive tract are distributed complementary to diamine oxidase[J]. Journal of Neural Transmission Supplementum, 1990, 32:345-349.
[19]  LI J Y, LU Y, HU S, et al. Preventive effect of glutamine on intestinal barrier dysfunction induced by severe trauma[J]. World Journal of Gastroenterology, 2002, 8(1):168-171.
[20]  VICARIO M, AMAT C, RIVERO M, et al. Dietary glutamine affects mucosal functions in rats with mild DSS-induced colitis[J]. The Journal of Nutrition, 2007, 137:1931-1937.
[21]  WU G, MEIER S A, KNABLE D A. Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs[J]. The Journal of Nutrition, 1996, 126:2578-2584.
[22]  MCDOWELL E M, ZHANG X M, DESNATI A M. Localization of healing tracheal wounds using bromodeoxyuridine immunohistochemistry[J]. Stain Technology, 1990, 65:25-29.
[23]  FAN M Z, STOLL B, JIANG R, et al. Enterocyte digestive enzyme activity along the crypt-villus and longitudinal axes in the neonatal pig small intestine[J]. Journal of Animal Science, 2001, 79:371-381.
[24]  吴秀清,王虹,孙梅,等.幼年大鼠内毒素血症时小肠上皮细胞凋亡及Caspase-3的表达[J].中国当代儿科杂志,2005,7(2):167-170.
[25]  ERBIL Y, OZTEZCAN S, GIRI?瘙塁 M, et al. The effect of glutamine on radiation-induced organ damage[J]. Life Science, 2005, 78(4):376-382.
[26]  SCHEPPACH W, DUSEL G, KUHN T, et al. Effect of L-glutamine and n-butyrate on the restitution of rat colonic mucosa after acid induced injury[J]. Gut, 1996, 38(6):875-885.
[27]  BRASSE-LAGNEL C G, LAVOINNE A M, HUSSON A S. Amino acid regulation of mammalian gene expression in the intestine[J]. Biochimie, 2010, 92(7):729-735.

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