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不同释放速度瘤胃调控剂对瘤胃亚急性酸中毒体外发酵参数的影响

DOI: 10.3969/j.issn.1006-267x.2011.09.021

Keywords: 瘤胃调控剂,释放速度,瘤胃亚急性酸中毒,发酵参数

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Abstract:

本试验通过在体外构建瘤胃亚急性酸中毒(SARA)模型,来研究不同释放速度的瘤胃调控剂对SARA体外发酵特性及牛链球菌数量的影响。选取3头育肥期并安装永久性瘤胃瘘管的宣汉黄牛,于晨饲2h后采集瘤胃内容物,滤液作为体外发酵液的接种物,通过添加玉米与白酒糟(1∶1)的方式诱导体外发生SARA。借助于构建成功的SARA体外模型,设置4个试验处理:对照组、6h完全释放组、8h完全释放组和10h完全释放组。在诱导SARA之后的0、2、4、6、8、10、12h取样测定瘤胃各发酵指标及牛链球菌的相对数量。结果表明,与对照顾组相比,各试验组瘤胃液pH均极显著升高(P<0.01),乙酸、丙酸、丁酸、乳酸和总挥发性脂肪酸(totalvolatilefattyacids,TVFA)浓度也显著降低(P<0.05)。结果显示,8h完全释放组不仅有效提高了瘤胃液pH,缓解了SARA状态,还对稳定瘤胃液环境和促进微生物的生长有显著效果。

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