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不同来源和剂量维生素C对建鲤生长性能和消化功能影响的比较研究

DOI: 10.3969/j.issn.1006-267x.2011.08.012

Keywords: 建鲤,乙基纤维素包被维生素C,维生素C磷酸酯钙盐,生长性能,消化功能

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Abstract:

本试验旨在研究不同来源和水平维生素C对建鲤生长性能和消化功能的影响。选用平均体重为(12.63±0.04)g的健康建鲤600尾,随机分为4组(每组设3个重复,每个重复50尾),分别饲喂添加不同来源[乙基纤维素包被维生素C(EC-AA)和维生素C磷酸酯钙盐(LAPP)]和剂量(75和150mg/kg)的维生素C的实用饲料,试验期为8周。结果表明,饲料维生素C水平为75mg/kg时,LAPP组建鲤在特定生长率(SGR)、摄食量(FI)、蛋白质沉积率(PPV)和脂肪沉积率(LPV)上均显著高于EC-AA组(P<0.05)。同时,LAPP饲料较EC-AA饲料显著促进建鲤肝胰脏和肠道生长发育,并显著提高肠道胰蛋白酶、糜蛋白酶和脂肪酶活性,各肠段皱襞高度,各肠段碱性磷酸酶(AKP)和γ-谷氨酰转肽酶(γ-GT)活性,前、后肠Na+,K+-ATP酶以及全肠肌酸激酶(CK)活性(P<0.05)。饲料维生素C水平为150mg/kg时,LAPP组和ECAA组建鲤在SGR、FI、PPV和LPV上差异不显著(P>0.05),但LAPP饲料较EC-AA饲料能显著提高建鲤肠道胰蛋白酶和糜蛋白酶活性,中、后肠皱襞高度,后肠AKP、前肠Na+,K+-ATP酶和各肠段γ-GT活性(P<0.05)。由此得出,在本试验条件下,LAPP的使用效果优于EC-AA,75mg/kg剂量下能更显著地提高建鲤生长性能和消化功能。

References

[1]  朱健,王建新.建鲤品种特性研究进展[J].浙江海洋学院学报:自然科学版,2004,23:52-55.
[2]  刘扬.饵料维生素C营养需要研究[D].硕士学位论文.雅安:四川农业大学,2001:1-60.
[3]  王文辉,王吉桥,程鑫,等.不同剂型维生素C对黄颡鱼生长和几种免疫指标的影响[J].中国水产科学,2006,13(6):951-958.
[4]  赵玉娟,李如华,刘慧文.牛肉样品中维生素C的HPLC测定[J].现代仪器,2005(3):32-33.
[5]  LIU P R, PLUMB J A, GUERIN M, et al. Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds[J]. Diseases of Aquatic Organisms, 1989, 7:191-194.
[6]  BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72:248-254.
[7]  HUMMEL B C W. A modified spectrophotometric determinations of chymotrypsin, trypsin, and thrombin[J]. Canadian Journal of Biochemistry and Physiology, 1959, 37:1393-1399.
[8]  FURNEA M, HIDALGOA M C,LOPEZ A.Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss.A comparative study[J]. Aquaculture, 2005, 250:391-398.
[9]  REFSTIE S, LANDSVERK T, BAKKE-MCKELLEP  M, et al. Digestive capacity, intestinal morphology, and microflora of 1-year and 2-year old Atlantic cod (Gadus morhua) fed standard or bioprocessed soybean meal[J]. Aquaculture, 2006, 261:269-284.
[10]  MCCORMIC S D. Methods for nonlethal gill biopsy and measurements of Na+,K+-ATPase[J]. Canadian Journal of Fisheries and Aquatic Science, 1993, 50:656-658.
[11]  WILSON R P, POE W E, ROBINSON E H. Evaluation of L-ascorbyl-2-polyphosphate (AsPP) as a dietary ascorbic acid source for channel catfish[J]. Aquaculture, 1989, 81:129-136.
[12]  冷向军,王道尊,李小勤.青鱼鱼种饲料中不同剂型维生素C适宜添加量的研究[J].四川农业大学学报,2002,20(2):141-143.
[13]  PEDERSEN H E, SISSONS J W. Effect of antigenic soyabean protein on the physiology and morphology of the gut in the preruminant calf[J]. Canadian Journal of Animal Science, 1984, 64:183-184.
[14]  FARHANGI M, CARTER C G, HARDY R W. Growth, physiological and immunological responses of rainbow trout (Oncorhynchus mykiss) to different dietary inclusion levels of dehulled lupin (Lupinus angustifolius)[J]. Aquaculture Research, 2001, 32:329-340.
[15]  VILLANUEVA J, VANACORE R, GOICOECHEA O, et al. Intestinal alkaline phosphatase of the fish Cyprinus carpio: regional distribution and membrane association[J]. Journal of Experimental Zoology, 1997, 279:347-355.
[16]  LIN Y, ZHOU X Q. Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J]. Aquaculture, 2006, 256:389-394.
[17]  BELL J G, BUDDINGTON R K, WALTON M J, et al. Study on the putative role of gamma-glutamyl transpeptidase in intestinal transport of amino acids in Atlantic salmon[J]. Journal of Compare Physiology, 1987, 157(2):161-169.
[18]  WILSON R P. Absence of ascorbic acid synthesis in channel catfish Ictalurus punctatus and blue catfish Ictalurus frucatus[J]. Comparative Biochemistry Physiology Part B, 1973, 46:635-638.
[19]  艾庆辉,麦康森,王正丽,等.维生素C对鱼类营养生理和免疫作用的研究进展[J].水产学报,2005,29(6):857-861.
[20]  SHIAU S Y, HSU T S. Stability of ascorbic acid in shrimp feed during analysis[J]. Nippon Suisan Gakkaishi, 1993, 59:1535-1537.
[21]  林仕梅,罗莉,叶元土,等.不同剂型VC对草鱼生长的影响[J].粮食与饲料工业,2003,4:30-32.
[22]  KUMULU M, JONES D A. The effect of live and artificial diets on growth, survival, and tripsin activity in larvae of Penaeus indicus[J]. Journal of the World Aquaculture Society, 1995, 26:406-415.
[23]  周秀菊,刘党生,蒋宇扬.肌酸激酶结构与功能研究进展[J].沈阳医科大学学报,2002,19(5):386.

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