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叔丁基对苯二酚对热应激小鼠肝脏氧化损伤的缓解作用

DOI: 10.3969/j.issn.1006-267x.2014.09.041

Keywords: 叔丁基对苯二酚,肝脏,热应激,氧化损伤

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Abstract:

本试验旨在研究叔丁基对苯二酚(tBHQ)对热应激小鼠肝脏氧化损伤的影响。试验选用50只6周龄ICR雄性小鼠随机分为2组,每组25只,tBHQ(-)组饲喂基础饲粮,tBHQ(+)组饲喂基础饲粮中添加1%tBHQ的试验饲粮。饲喂14d后,小鼠每天进行42℃2h热应激处理,连续8d。在热应激之前(热应激0d)和热应激1、2、4、8d,每组各取5只小鼠处死,采集肝脏,测定肝脏重量和抗氧化指标,免疫组织化学方法检测转录因子E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)蛋白表达,实时定量PCR法测定Nrf2及其调控表达基因的表达量。结果表明:1)tBHQ(-)组肝脏指数在热应激4d恢复正常水平,而tBHQ(+)组在热应激2d即恢复正常水平。2)与tBHQ(-)组相比,热应激0d,tBHQ(+)组肝脏总超氧化物歧化酶(T-SOD)活力显著升高(P<0.05),在热应激4d时,还原型谷胱甘肽含量以及T-SOD、铜,锌超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活力显著降低(P<0.05)。3)热应激2d,tBHQ(-)组比tBHQ(+)组肝脏损伤情况更为严重。4)热应激0d,tBHQ(-)组未检测到Nrf2蛋白在肝脏组织表达,热应激后各时间点都检测到Nrf2蛋白的表达,而tBHQ(+)组在各阶段均有表达;tBHQ(-)组在热应激0、1、4和8d都检测到HO-1蛋白的表达,而tBHQ(+)组在热应激0、1和2d时检测到了HO-1蛋白的表达。5)与tBHQ(-)组相比,tBHQ(+)组多个Kelch样ECH相关蛋白(Keap1)-Nrf2/ARE信号通路相关基因表达量均显著升高(P<0.05)。综上所述,饲粮添加1%tBHQ对热应激造成的小鼠肝脏氧化损伤能够起到一定的缓解作用。

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