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几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

, PP. 120-126

Keywords: 原甲藻属,28SrDNA,系统分析

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Abstract:

对7株赤潮原甲藻28SrDNA5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28SrDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28SrDNA扩增序列长度为950~958bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrummicans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28SrDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.

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