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健康鸡源肺炎克雷伯菌检测到质粒介导喹诺酮耐药qnrA基因

DOI: 10.7671/j.issn.1001-411X.2012.02.030, PP. 259-263

Keywords: 健康动物,肺炎克雷伯菌,qnrA1基因,整合子

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Abstract:

通过PCR技术检测195株健康动物肠杆菌中qnrA基因的流行情况;琼脂稀释法对qnrA阳性菌株进行8种抗菌药物的药物敏感性试验;质粒接合转移试验研究qnrA的可转移性;Southern杂交对qnrA基因进行定位;PCR检测qnrA阳性菌中Ⅰ类整合酶intI基因的携带情况.结果表明,195株肠杆菌中检出1株qnrA阳性的肺炎克雷伯菌KlebsiellapneumoniaeGDKA1,经Blast鉴定为qnrA1.药物敏感性试验表明GDKA1对磺胺甲恶唑、氨苄西林、大观霉素、氯霉素、四环素耐药,对环丙沙星(MIC=0.019mg·L-1),头孢噻肟(MIC=0.047mg·L-1)表现敏感,对萘啶酸(MIC=12mg·L-1)表现为部分敏感.接合试验表明qnrA1位于可转移的质粒上,且可以通过接合试验水平传播.接合子TGDKA1对喹诺酮类药物的敏感性比大肠埃希菌EscherichiacoliJ53AzR稍高,但远低于GDKA1.Southern杂交进一步证实了qnrA1基因位于质粒上.TGDKA1的质粒扩增出intI1,表明qnrA1阳性菌株携带I类整合子.可见,qnrA1基因已在健康鸡源肺炎克雷伯菌中出现且同时携带I类整合子位于质粒上,整合子灵活多样的存在方式和传播方式为qnrA基因的传播提供了便利条件,整合子可能携带qnrA基因通过食物链传播给人,因此健康动物共生菌携带qnrA1基因值得注意.

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