全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

用二维相关红外光谱研究蛋白质分子的热动力学过程

Keywords: 二维相关分析红外光谱热动力学牛血清白蛋白傅立叶变换

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用傅立叶变化红外(FT-IR)二维样本和波数相关光谱研究了水溶液中牛血清白蛋白(Bovineserumalbumin)的热动力学过程.二维样本相关分析的结果给出了牛血清白蛋白随温度变化的趋势,表明牛血清白蛋白剧烈的结构变化开始于66℃;而二维波数相关分析提供了在升温过程中牛血清白蛋白结构上变化和各结构变化次序的细节.研究显示了二维相关光谱在蛋白质热变性分析中的重大作用.

References

[1]  Noda I. Generalized two-dimensional correlation method applicable to infrared, Raman and other types of spectroscopy [J]. Appl. Spectrosc., 1993, 47 ( 9 ) : 1329-1336.
[2]  REN Y, Murakami T, Nishioka T, et al. Two-dimensional near-infrared correlation spectroscopy studies of compatible polymer blends: composition-dependent spectral variations of blends of atactic polystyrene and poly ( 2,6-dimethyl-1, 4-phenylene ether) [J]. J. Phys. Chem. , 2000, 104( 4 ) :679-690.
[3]  WU Y Q, Yukihiro O. Two-dimensional infrared correlation spectroscopy studies of proteins in aqueous solutions[J]. J.Infrared. Millim. Waves, 2003, 22(3) : 161-168.
[4]  Sasic S, Muszynski A, Ozaki Y. A new possibility of the generalized two - dimensional correlation spectroscopy. 1.sample - sample correlation spectroscopy [ J ]. J. Phys.Chem. , 2000, A104 ( 27 ) : 6380-6387.
[5]  Ruegg M, Moor U, Blanc B. A calorimetric study of the thermal denaturation of whey proteins in simulated milk ultrafiltrate[J]. J. Dairy Res. , 1977, 44: 509-520.
[6]  Itoh T, Wada Y, Nakanishi T. Differential thermal analysis of milk proteins[J]. Agr. Biol. Chem., 1976, 40: 1083-1086.
[7]  Hu X, Rodgers K R, Mukerji I, et al. New light on allostery: dynamic resonance Raman spectroscopy of hemoglobin kempsey [ J ]. Biochemistry, 1999, 38: 3462-3467.
[8]  Panick G, Winter R. Press-induced unfolding/refolding of Ribonuclease A: static and kinetic Fourier transform infrared spectroscopy study[J]. Biochemistry, 2000, 39: 1862-1869.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133