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华北农学报 1993

Somaticembryogenesis,syntheticseedpreparationandplantregenerationinApiumgraveolensvar.Dulcepers

DOI: 10.7668/hbnxb.1993.S2.008, PP. 45-47

曹鸣庆,刘凡,蒋涛

Keywords: Apiumgraveolensvar.Dulcepers.,cellculture,somaticembryogenesis,synthet-icseeds

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Abstract:

OntheMSmediumsupplementedwith2ppm2,4-D,calliwereinducedafter4-6weeksfromthepetiolesofanAmericanceleryplant（Apiumgraveolensvar.Dulcepers.cv.Florida）.Suspensionculturewasstartedfromthecalliinahormone-freeliquidMSmediumonagyratoryshakerat110rpm,andkeptat26℃.Tostimulatecelldivisionanddedifferentiation,thesubcultureswereconductedfor7dayseachonthesamemedium.Theliquidsuspensioncontainingsinglecells,cellaggregates,andsomaticembryosindifferentstageswerescreened2-3weekslaterand1.0-1.5mmsomaticembryoswereobtained.Theseembryoswereencapsulatedwithsodiumalginatebydropping-beadmethodandsolidifiedwith0.1molCaCl2,.Thesesyntheticseedsgerminatedanddevelopedwellintoseedlingsinthesterilizedvermiculitesubstrate.

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