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DT40细胞slgMλ轻链基因敲除载体的构建

DOI: 10.7668/hbnxb.2009.03.001, PP. 1-6

Keywords: slgM&lambda,轻链,&beta,-actin启动子,基因敲除,同源重组

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Abstract:

从鸡B淋巴DT40细胞系中克隆β-actin启动子,替换pCDNA3.1(+)载体中的SV40启动子,构建β-octin启动子驱动的Neomycin抗性基因表达框.将克隆的slgMλ轻链基因两侧各约2kb的序列插入到抗性表达框的两侧作为同源臂.PCR、酶切以及测序结果表明成功构建了靶向slgMλ轻链基因的置换型打靶载体pCDNA-act-neo-HR.为建立slgMλ轻链基因敲除的DT40细胞模型,探究slgMλ轻链在IBDV感染DT40细胞过程中的作用奠定了基础.

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