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华北农学报 1987

谷蛋白和醇溶蛋白的电泳分析及小麦品种鉴定

DOI: 10.3321/j.issn:1000-7091.1987.04.003, PP. 16-21

王洪新,胡志昂

Keywords: 电泳分析,小麦品种鉴定

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Abstract:

应用不同浓度梯度的聚丙烯酰胺凝胺SDS电泳比较分析了18个小麦品种的、用不同提取方法制备的醇溶蛋白和谷蛋白。结果表明，经1M脲素提取掉多数醇溶蛋白、清蛋白及其它醇溶成分后的小麦样品，用加有2%SDS和5%β-巯基乙醇的裂解缓冲液提取的高分子量谷蛋白和醇溶蛋白，在8-12%梯度的SDS胶中显示出良好的分辨率和极大的多态性，适于小麦品种鉴定。用裂鲜缓冲液直接提取半粒小麦的全蛋白可用于逐粒品种鉴别。

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