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小麦转录因子TaSIM的原核表达分析

DOI: 10.3969/j.issn.1000-7091.2013.02.011, PP. 60-62

Keywords: 小麦,TaSIM,原核表达

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Abstract:

为了进一步研究TaSIM基因的功能,以pJET1.2-TaSIM质粒为模板,PCR扩增TaSIM基因的cDNA编码区,构建了该基因的原核表达载体pET-28a-TaSIM,经菌液PCR和测序鉴定后转化到大肠杆菌BL21(DE3)中。结果表明,在大肠杆菌BL21(DE3)菌株中成功表达了与标签蛋白融合的TaSIM蛋白,大小约为33kDa。SDS-PAGE电泳分析表明,最佳诱导表达条件为0.5mmol/LIPTG在37℃下诱导2h。

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