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小麦TaMSR基因的克隆与表达分析

DOI: 10.7668/hbnxb.2014.04.006, PP. 32-36

Keywords: 小麦,TaMSR基因,表达分析,染色体定位

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Abstract:

为了发掘小麦育性基因,采用同源克隆的方法,从小麦花药中克隆了3个与拟南芥AtMS2和水稻OsMS2基因同源的cDNA序列。TaMSR-1、TaMSR-2和TaMSR-3分别具有1842,1824,1830bp的开放阅读框,各自编码613,607,609个氨基酸。推导的蛋白质序列中包含2个保守区:NAD_binding和Sterile保守功能域。氨基酸序列分析发现,TaMSR与水稻OsMSR2和拟南芥AtMSR2具有较高的相似性。采用基因特异定位引物PCR技术对中国春缺失系材料进行扩增,将TaMSR-1、TaMSR-2和TaMSR-3基因分别定位于4AS、4DL和4BL染色体上。半定量RT-PCR分析表明,TaMSR基因是花药组织特异表达的基因。这些结果说明,TaMSR基因可能在小麦花药发育过程中起重要作用。

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