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甘蓝型油菜LPAT4基因克隆及其生物信息学分析

DOI: 10.7668/hbnxb.2014.03.011, PP. 52-58

Keywords: LPAT4,甘蓝型油菜,同源建模分析,保守结构域,SNP

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Abstract:

为进一步研究油菜LPAT4基因,通过检索与拟南芥LPAT4序列相似度较高的油菜EST序列,拼接后与白菜和甘蓝的LPAT4序列进行同源比对,设计一对引物,从甘蓝型油菜湘油15中扩增全长LPAT4CDs序列。共获得2条CDs序列,它们的长度分别为1143,1140bp,分别命名为BnLPAT4-1和BnLPAT4-2。BnLPAT4-1编码380个氨基酸,所得蛋白质的相对分子质量和等电点分别为43.29kDa和8.74;BnLPAT4-2编码379个氨基酸,所得蛋白质的相对分子质量和等电点分别为43.16kDa和8.93。他们均具有LPLAT_LCLAT1样结构域,同属于LPLAT超基因家族。比较所用油菜序列SNP分析显示,LPAT4共存在41个多态性位点,其中单变异位点7个,简约信息位点34个。这41个多态性位点中,引起同义变异的位点为23个,引起取代变异的位点18个,共导致16个氨基酸发生改变。分析表明,BnLPAT4-1和BnLPAT4-2为甘蓝型油菜湘油15号LPAT4基因的2个拷贝,从序列组成、理化性质、保守结构域以及同源建模4个方面来看,它们均具备LPLAT超基因家族的特征,为该基因家族的2个成员。

References

[1]  张 垚,李云昌,梅德圣,等.油菜油脂研究进展[J].植物学通报,2007,24(4):435-443.
[2]  Vigeolas H,Waldeck P,Zank T,et al.Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter[J].Plant Biotechnol J,2007,5(3):431-441.
[3]  David C,Taylor Y Z A K,Dennis L,et al.Molecular modification of triacylglycerol accumulation by over-expression of DGAT1 to produce canola with increased seed oil content under field conditions[J].Botany,2009,87:533-543.
[4]  Burns M J,Barnes S R,Bowman J G,et al.QTL analysis of an intervarietal set of substitution lines in Brassica napus:Seed oil content and fatty acid composition[J].Heredity (Edinb),2003,90(1):39-48.
[5]  Chai G,Bai Z,Wei F,et al.Brassica GLABRA2 genes: analysis of function related to seed oil content and development of functional markers[J].TAG Theoretical and Applied Genetics,2010,120(8):1597.
[6]  Zou J,Katavic V,Giblin E M,et al.Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene[J].Plant Cell,1997,9(6):909-923.
[7]  Rao S S,Hildebrand D.Changes in oil content of transgenic soybeans expressing the yeast SLC1 gene[J].Lipids,2009,44(10):945-951.
[8]  Maisonneuve S,Bessoule J J,Lessire R,et al.Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis1 [J].Plant Physiol,2010,152(2):670-684.
[9]  戚维聪.油菜发育种子中油脂积累与Kennedy途径酶活性的关系研究[D].南京:南京农业大学,2008.
[10]  陈四龙,黄家权,雷 永,等.花生溶血磷脂酸酰基转移酶基因的克隆与表达分析[J].作物学报,2012,38(2):245-255.
[11]  Kim H U,Li Y,Huang A H.Ubiquitous and endoplasmic reticulum-located lysophosphatidyl acyltransferase,LPAT2, is essential for female but not male gametophyte development in Arabidopsis[J].Plant Cell,2005,17(4):1073-1089.
[12]  Arroyo-Caro J M,Chileh T,Kazachkov M,et al.The multigene family of lysophosphatidateacyltransferase (LPAT)- related enzymes in Ricinus communis.Cloning and molecular characterization of two LPAT genes that are expressed in castor seeds[J].Plant Sci,2013,199-200:29-40.
[13]  Hanke C,Wolter F P,Coleman J,et al.A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase mutant[J].Eur J Biochem,1995,232(3):806-810.
[14]  Knutzon D S,Lardizabal K D,Nelsen J S,et al.Cloning of a coconut endosperm cDNA encoding a 1-acyl-sn-glycerol-3-phosphate acyltransferase that accepts medium-chain-length substrates[J].Plant Physiol,1995,109(3):999-1006.
[15]  Brown A P,Coleman J,Tommey A M,et al.Isolation and characterisation of a maize cDNA that complements a 1-acyl sn-glycer-ol-3-phosphate acyltransferase mutant of Escherichia coli and encodes a protein which has similarities to other acyltransferases[J].Plant Mol Biol,1994,26(1):211-223.
[16]  Brown A P,Carnaby S,Brough C,et al.Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification,acyl selectivity and functional replacement of the Escherichia coli plsC gene[J].Biochem J,2002,364:795-805.
[17]  Brown A P,Kroon J T,Swarbreck D,et al.Tissue-specific whole transcriptome sequencing in castor,directed at understanding triacylglycerol lipid biosynthetic pathways[J].PLoS One,2012,7(2):1-13.
[18]  姚行成.芸薹属栽培种与野生种Brassicamaurorum的种间杂交及基因组关系[D].武汉: 华中农业大学,2010.
[19]  Chen X,Li X E,Zhang B,et al.Detection and genotyping of restriction fragment associated polymorphisms in polyploid crops with a pseudo-reference sequence: a case study in allotetraploid Brassica napus[J].BMC Genomics,2013,14(1):346.
[20]  Kazachkova N,Fahleson J,Meijer J.Establishment of the amplified fragment length polymorphism (AFLP) technique for genotyping of pollen beetle (Meligethesaeneus)-a noxious insect pest on oilseed rape (Brassica napus)[J].MolBiol Rep,2004,31(1):37-42.
[21]  Bus A,Hecht J,Huettel B,et al.High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing[J].BMC Genomics,2012,13:281.
[22]  Durstewitz G,Polley A,Plieske J,et al.SNP discovery by amplicon sequencing and multiplex SNP genotyping in the allopolyploid species Brassica napus[J].Genome,2010,53(11):948-956.
[23]  Howell E C,Kearsey M J,Jones G H,et al.A and C genome distinction and chromosome identification in Brassica napus by sequential fluorescence in situ hybridization and genomic in situ hybridization[J].Genetics,2008,180(4):1849-1857.

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