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鸡贫血病毒VP1基因的克隆及其在大肠杆菌中的表达

DOI: 10.3321/j.issn:1000-7091.2006.05.013, PP. 50-53

Keywords: 鸡贫血病毒,VP1基因,克隆,表达

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Abstract:

根据鸡贫血病毒Cux1株基因组序列设计了一对引物,通过PCR扩增出VP1基因。将扩增出的片段克隆到pGMT载体上,通过测序分析证实该片段与鸡贫血病毒Cux1株的VP1基因序列一致。然后将克隆化VP1基因亚克隆到PET32a(+)载体上并进行原核表达。SDSPAGE和WesternBlot分析表明,一个分子质量为70.4kDa的重组蛋白获得了成功表达,并且鸡贫血病毒阳性血清能和重组蛋白发生反应。

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