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TcLr35小麦β(1,3;1,4)葡聚糖苷酶基因cDNA全长的克隆及分析

DOI: 10.7668/hbnxb.2010.04.005, PP. 25-29

Keywords: 小麦,&beta,(1,3,1,4)葡聚糖苷酶,基因克隆,序列分析

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Abstract:

根据已发表的植物β(1,3;1,4)葡聚糖苷酶基因设计引物,利用RT-PCR和RACE技术,从被小麦叶锈菌诱导的小麦抗叶锈病基因近等基因系材料TcLr3中获得一个β(1,3;1,4)葡聚糖苷酶基因cDNA全长,命名为PR34,该基因全长序列为1416bp,具有一个编码33个氨基酸的开放阅读框(ORF),其起始密码子ATG位于13bp处,终止密码子TAG位于101bp处,3′末端有20bp的poly(A)尾,379bp的3′非翻译区.与GenBank中小麦、大麦等多个葡聚糖苷酶基因具有较高同源性;对其推断的氨基酸序列进行分析,含有Glyco_hydro_17保守结构域,为葡聚糖苷酶基因蛋白结构域.Southern杂交显示,该基因在TcLr3小麦基因组中为低拷贝.

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