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津田芜菁泛素结合酶BrUBC11基因的克隆及表达分析

DOI: 10.7668/hbnxb.2015.01.016, PP. 97-102

Keywords: 津田芜菁,UBC11,基因克隆,表达分析

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Abstract:

为阐释津田芜菁BrUBC11基因的表达特性,克隆了津田芜菁UBC11基因的全长cDNA序列,命名为BrUBC11,GenBank登录号为KM396887。其cDNA全长685bp,ORF区全长447bp,编码148个氨基酸的开放阅读框;亚细胞定位结果显示,BrUBC11-GFP定位于细胞核内,表明BrUBC11蛋白可能在细胞核中发挥其功能。荧光定量PCR检测BrUBC11在芜菁不同组织中的表达结果表明,该基因在花瓣中表达量最高,花蕾中次之,具有组织特异性。而且BrUBC11在芜菁白色根皮中的表达受长波紫外线(UV-A)诱导。研究结果显示,在芜菁中,UBC11属于多功能基因,具有潜在的参与花发育和UV-A信号转导相关途径的功能。

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