全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

氨基酸对类弹性蛋白多肽产量的影响

DOI: 10.11830/ISSN.1000-5013.2013.05.0542

Keywords: 类弹性蛋白多肽, 条件优化, 氨基酸, 响应面设计

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用谷氨酰胺来确定氨基酸初始添加浓度,分析各种氨基酸对类弹性蛋白多肽[KV8F-40]产量的影响.结果表明:苏氨酸、精氨酸和半胱氨酸都能较大提高产量,其中以苏氨酸最为明显,产量可达126.9 mg?L-1,比对照增加54.0%.利用响应面设计对上述3种氨基酸进行3因素3水平实验,经优化后所得理论最大产量为100.3 mg?L-1.经实验验证,所得实验值为103.1 mg?L-1,与理论值吻合,但其产量低于单个氨基酸.交互作用分析表明:3种对产量有促进作用的氨基酸之间没有互作或者存在拮抗作用.

References

[1]  RYU B Y,SOHN J S,HESS M,et al.Synthesis and anti-cancer efficacy of rapid hydrolysed water-soluble paclitaxel pro-drugs[J].Biomaterials Science,2008,19(3):311-324.
[2]  FUJITA Y,MIE M,KOBATAKE E.Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain[J].Biomaterials,2009,30(20):3450-3457.
[3]  KORIA P,YAGI H,KITAGAWA Y,et al.Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds[J].Proceedings of the National Academy of Sciences(USA),2011,108(3):1034-1039.
[4]  DOMINIC C.CHOW,MATTHEW R D,KIMBERLY T C,et al.Ultra-high expression of a thermally responsive recombinant fusion protein in E.coli[J].Biotechnology Progress,2006,22(3):638-646.
[5]  黄凯宗,李晶晶,李巍,等.类弹性蛋白多肽的从头设计、非色谱纯化及盐效应[J].生物工程学报,2011,27(4):653-658.
[6]  MEYER D E,CHILKOTI A.Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: Examples from the elastin-like polypeptide system[J].Biomacromolecules,2002,3(2):357-367.
[7]  张卫兰,陶国庆,刘刚,等.氨基酸对大肠杆菌BL21产嘌呤核苷磷酸化酶的影响[J].化学与生物工程,2010,27(2):53-56.
[8]  HARCUM S W,RAMIREZ D M,BENTLEY W E.Optimal nutrient feed policies for heterologous protein production [J].Applied Biochemical and Biotechnology,1992,34/35(1):161-173.
[9]  CHU H S,PARK J E,KIM D M,et al. The effects of supplementing specific amino acids on the expression of elastin-like polypeptides(ELPs)[J].Protein Expression and Purification,2010,74(2):298-303.
[10]  张文超,陈长华,李素霞,等.Mg2+和氨基酸对重组大肠杆菌BL21(DE3)生长及羧肽酶原B表达的影响[J].中国生物制品学杂志,2006,19(1):81-83.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133