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嗜肺军团菌momp原核重组质粒的构建和表达

Keywords: 嗜肺军团,momp基因,表达,纯化

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Abstract:

目的构建嗜肺军团菌momp原核重组质粒,并纯化重组蛋白。方法以嗜肺军团菌LP1型DNA为模板,PCR扩增得到momp基因,定向克隆至原核载体PET32a(+)中,经酶切及测序鉴定正确后,转化入大肠杆菌BL21中,用IPTG诱导并用SDS-PAGE电泳进行分析,其产物用亲和层析法进行纯化。结果扩增出831bp的momp基因,构建重组质粒pET-momp,诱导表达及纯化出50KD的蛋白。结论成功构建momp基因的原核表达载体并得到高效表达。

References

[1]  张莉,张雷.嗜肺军团菌主要外膜蛋白DNA疫苗的免疫保护性研究[J].西部医学,2006,18(6):694-696.
[2]  Ricketts KD, Joseph CA.The impact of new guidelines in Europe for the control and prevention of travel-associated Legionnaires' disease[J]. Int J Hyg Environ Health,2006,209(6):547-552.
[3]  Karbyshev GL, Terent'ev AN,Verkina LM,et al. Serologic diagnostics of Legionella infection[J]. Zh Mikrobiol Epidemiol Immunobiol,2008(2):46-50.
[4]  Gaia V, Fry NK, Harrison TG, et al. Sequence-based typing of Legionella pneumophila serogroup 1 offers the potenti al for trueportability in legionellosi s outbreak investigation[J].Clin Microbiol,2003,41(7):2932-2939.
[5]  Ricci ML, Torosantucci A, Scaturro M,et al. Induction of protective immunity by Legionella pneumophila flagellum in an A/J mouse model [J]. Vaccine,2005,23(40):4811-4820.
[6]  刘向伟,张浩杰.重组鹦鹉热衣原体主要外膜蛋白的抗原性研究[J].生命科学研究,2002,6(3):267-268.
[7]  Yoon WS, Park SH, Park YK, et al. Comparison of responses elicited by immunization with a Legionella species common lipoprotein delivered as naked DNA or recombinant protein.[J]. DNA Cell Biol,2002,21(2):99-107.
[8]  Gaia V, Fry NK. Consensus s equence-based scheme for epidemiological typing of clinical and environment al isol at es of L egionella pneumophila[J]. J Clin Microbiol,2005,43(5):2047-2052.

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