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华西医学  2010 

多柔比星致兔心功能变化的实验研究

, PP. 2167-2170

Keywords: 多柔比星,心脏毒性,彩色多普勒超声心动图,脑钠肽,

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Abstract:

【】 目的 采用多柔比星(doxorubicin,DOX)制备心肌损伤动物模型,评价各种检测心功能变化方法的意义。 方法 14只新西兰大白兔,DOX耳缘静脉注射,每周3mg/kg,共10周。分别于给药前、第4周末及实验结束时测定血清肌钙蛋白Ⅰ(cTnI)和脑钠肽(BNP)水平,彩色多普勒超声心动图检测心功能变化,并观察心肌组织病理形态学改变及心肌细胞凋亡情况。 结果 使用DOX前后对比,血清cTnI和BNP浓度升高(P<0.05);左室射血分数(LVEF)和左室短轴缩短率(LVFS)下降(P<0.05);心肌组织病理显示心肌细胞出现不同程度的空泡变性与水肿,细胞间隙明显增宽,大量炎性细胞浸润。心肌细胞凋亡明显增加。 结论 结合心脏超声检查和血清cTnI、BNP指标检测可判断心肌损伤程度。?【Abstract】 Objective Toobservethechangesofheartfunctioncausedbydoxorubicininrabbits. Methods Atotalof14New-Zealandwhiterabbitswereintravenous-injectedwithdoxorubicinwithadosageof3mg/kgintravenouslyonceaweek,andtheaccumulativedosewas30mg/kg.Beforethemedicationandatthe4thand10thweekendafterthemedication,theserumlevelsofcardiactroponinI(cTnI)andbrainnatriureticpeptide(BNP)weremeasured;leftventricularejectionfunction(LVEF)andleftventricularfractionalshortening(LVFS)wereperformedontherabbitsrespectively.Atthe10thweekend,thepathologicalchangesofcardiactissueandtheapoptosisofmyocardialcellweredetected. Results ThelevelsofcTnIandBNPsignificantlyincreased(P<0.05),andtheLVEFandLVFSmarkedlydecreased(P<0.05)aftertheadministrationofdoxorubicin.Unevenvacuolardegenerationandedemaofcardiocytescouldbeobservedwithawidecellspacesandinflammatorycellinfiltrationinthehistopathologicalslices. Conclusion ThecombinedapplicationofheartsonographywiththedetectionoftheserumlevelsofcTnIandBNPcanevaluatethedegreeofmyocardialdamageoftherabbitsmodelsverywell.

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