%0 Journal Article
%T 多柔比星致兔心功能变化的实验研究
%A 车菲菲
%A 徐娟
%A 刘瑜
%A 徐才刚
%J 华西医学
%P 2167-2170
%D 2010
%X 【】　目的　采用多柔比星（doxorubicin，DOX）制备心肌损伤动物模型，评价各种检测心功能变化方法的意义。　方法　14只新西兰大白兔，DOX耳缘静脉注射，每周3mg/kg，共10周。分别于给药前、第4周末及实验结束时测定血清肌钙蛋白Ⅰ（cTnI）和脑钠肽（BNP）水平，彩色多普勒超声心动图检测心功能变化，并观察心肌组织病理形态学改变及心肌细胞凋亡情况。　结果　使用DOX前后对比，血清cTnI和BNP浓度升高（P<0.05）；左室射血分数（LVEF）和左室短轴缩短率（LVFS）下降（P<0.05）；心肌组织病理显示心肌细胞出现不同程度的空泡变性与水肿，细胞间隙明显增宽，大量炎性细胞浸润。心肌细胞凋亡明显增加。　结论　结合心脏超声检查和血清cTnI、BNP指标检测可判断心肌损伤程度。？【Abstract】　Objective　Toobservethechangesofheartfunctioncausedbydoxorubicininrabbits.　Methods　Atotalof14New-Zealandwhiterabbitswereintravenous-injectedwithdoxorubicinwithadosageof3mg/kgintravenouslyonceaweek,andtheaccumulativedosewas30mg/kg.Beforethemedicationandatthe4thand10thweekendafterthemedication,theserumlevelsofcardiactroponinI(cTnI)andbrainnatriureticpeptide(BNP)weremeasured;leftventricularejectionfunction(LVEF)andleftventricularfractionalshortening(LVFS)wereperformedontherabbitsrespectively.Atthe10thweekend,thepathologicalchangesofcardiactissueandtheapoptosisofmyocardialcellweredetected.　Results　ThelevelsofcTnIandBNPsignificantlyincreased(P<0.05),andtheLVEFandLVFSmarkedlydecreased(P<0.05)aftertheadministrationofdoxorubicin.Unevenvacuolardegenerationandedemaofcardiocytescouldbeobservedwithawidecellspacesandinflammatorycellinfiltrationinthehistopathologicalslices.　Conclusion　ThecombinedapplicationofheartsonographywiththedetectionoftheserumlevelsofcTnIandBNPcanevaluatethedegreeofmyocardialdamageoftherabbitsmodelsverywell.
%K 多柔比星
%K 心脏毒性
%K 彩色多普勒超声心动图
%K 脑钠肽
%K 兔
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201012011