全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
华西医学  2011 

小鼠肾炎模型的制备及双氢青蒿素对其炎症因子释放的影响

, PP. 1028-1031

Keywords: 肾炎模型,双氢青蒿素,脂多糖,肿瘤坏死因子-α,白细胞介素-6,小鼠

Full-Text   Cite this paper   Add to My Lib

Abstract:

【】 目的 制备小鼠肾炎模型并观察双氢青蒿素(dihydroartemisinine,DHA)对模型小鼠细胞因子肿瘤坏死因子-α(tunornecrosisfactor,TNF-α)和白细胞介素-6(interleukin-6,IL-6)的影响以及小鼠肾脏的病理变化。 方法 取雄性昆明种小鼠120只,随机分为正常对照组、脂多糖(lipopolysaccharides,LPS)组、LPS+肾匀浆组及DHA治疗组;分别于12、24、48h取血,酶联免疫吸附试验检测血清中TNF-α和IL-6的含量,苏木精-伊红染色法观察小鼠肾脏的病理变化。 结果 造模48hLPS+肾匀浆组小鼠肾小球出现炎性细胞浸润,而正常对照组未见异常;LPS组及DHA治疗组仅有轻微的病理改变。LPS刺激使小鼠血清TNF-α和IL-6含量高于正常水平(P0.05)。 结论 运用改良的造模方法LPS+肾匀浆建立肾炎模型效果良好;DHA可以调节模型小鼠炎症因子TNF-α和IL-6的释放,具有一定的改善模型小鼠肾炎症状的作用。【Abstract】 Objective Toestablishmicenephritismodels,detecttheserumlevelchangesofcytokinestumornecrosisfactorα(TNF-α)andinterleukin-6(IL-6)inthemodelmicetreatedbyDihydroartemisinin(DHA),andobservethephysiologicalchangesofthemicekidneys. Methods OnehundredandtwentymaleKunmingmicewererandomlydividedinto4groupscontrolgroup,lipopolysaccharides(LPS)group,LPSpluskidneyhomogenategroup,andDHAtreatedgroup.ThelevelofcytokinesTNF-αandIL-6intheserumweredetectedbyenzyme-linkedimmunoabsordentasseyathour12,24,and48,respectively.Pathologicalchangeswereobservedbyhematoxylineosinstaining. Results Atthetimehour48aftertheestablishmentofthemodel,inflammatorycellinfiltrationwasobservedintheglomerulusoftheLPSpluskidneyhomogenategroup,butnoabnormalitywasfoundinthecontrolgroup.TherewereonlyslightpathologicalchangesinmicemodelsoftheLPSgroupandtheDHAtreatedgroup.TheserumlevelofTNF-αandIL-6increasedremarkablyafterthetreatmentofLPS(P0.05). Conclusion ThemodifiedLPSpluskidneyhomogenatehasagoodresultinmodelestablishing.DHAcanregulatethereleaseofTNF-αandIL-6inthemodelmice,andmayhavecertaingoodeffectsonamelioratingthenephritispathologicalchanges.

References

[1]   仲芳, 陈慧, 韩琳, 等. 姜黄素对内毒素所致肾脏炎症的抑制作用[J]. 中国中西医结合肾病杂志, 2009, 10(11): 948-951.
[2]   Zahner G, Schaper M, Panzer U, et al. Prostaglandin EP2 and EP4 receptors modulate expression of the chemokine CCL2 (MCP-1) in response to LPS-induced renal glomerular inflammation[J]. Biochem J, 2009, 422(3): 563-570.
[3]   Shui HA, Ka SM, Wu WM, et al. LPS-evoked IL-18 expression in mesangial cells plays a role in accelerating lupus nephritis[J]. Rheumatology, 2007, 46(8): 1277-1284.
[4]   Meyer-Schwesinger C, Dehde S, von Ruffer C, et al. Rho kinase inhibition attenuates LPS-induced renal failure in mice in part by attenuation of NF-kappa B p65 signaling[J]. Am J Physiol Renal Physiol, 2009, 296(5): 1088-1099.
[5]   苑同业, 梁弘钢, 梁冰, 等. 肾小球肾炎患儿治疗前后TNFα、sTNFR、IL-6 和IL-8水平变化[J]. 上海医学检验杂志, 2003, 18(5): 260.
[6]   段翠蓉, 李志辉. 白细胞介素-6与肾小球疾病的关系研究进展[J]. 中国当代医药, 2010, 17(16): 14-15.
[7]   刘云海, 杜光. 青蒿素等中药化学成分抗内毒素研究现状[J]. 医药导报, 2001, 20(8): 525-526.
[8]   梁爱华, 薛宝云, 王金华, 等. 青蒿琥酯对内毒素诱导的炎性因子合成的抑制作用的研究[J]. 中国中西医结合急救杂志, 2001, 8(5): 262.
[9]   王俊, 周红. 青蒿素对CpG DNA攻击小鼠保护作用的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(3): 290-293.
[10]   董妍君, 李卫东, 屠呦呦, 等. 双氢青蒿素对BXSB狼疮小鼠自身抗体产生、TNFα分泌及狼疮性肾炎病理改变的影响[J]. 中国中西医结合杂志, 2003, 23(9): 676-679.
[11]   赵桂芝, 聂淑琴, 杨庆, 等. 清脂胶囊对内毒素诱导的炎症介质水平的影响及时效关系[J]. 中国实验方剂学杂志, 2006, 12(11): 39-42.
[12]   Sabry A, Elbasyouni SR, Sheashaa HA, et al. Correlation between levels of TNF-alpha and IL-6 and hematological involvement in SLE Egyptian Patients with lupus nephritis[J].Int Urol Nephrol, 2006, 38(3-4): 731-737.
[13]   周立文, 贝文政, 王建才. 双氢青蒿素对恶性疟疾的疗效及免疫调节作用观察[J]. 热带病病与寄生虫学, 2008, 6(3): 138-139.
[14]   Chen H, Sun B, Pan S, et al. Dihydroartemisinin inhibits growth of pancreatic cancer cells in vitro and in vivo[J].Anticancer Drugs, 2009, 20(2): 131-140.
[15]   Noori S, Hassan ZM, Taghikhani M, et al. Dihydroartemisinin can inhibit calmodulin, calmodulin-dependent phosphodiesterase activity and stimulate cellular immune responses[J].Int Immunopharmacol, 2010, 10(2): 213-217.
[16]   张荣侠, 吕晶晶, 戴夕超, 等. 双氢青蒿素对辐射小鼠血液系统的保护作用[J]. 中国现代医药杂志, 2009, 11(11): 39-41.
[17]   李斌, 张乐之, 王俊, 等. 双氢青蒿素对CpG ODN诱导小鼠RAW264.7细胞释放细胞因子的影响[J]. 四川生理科学杂志, 2005, 27(4): 149-152.
[18]   梁爱华, 薛宝云, 李春英, 等. 青蒿琥酯对内毒素诱导的一氧化氮合成的抑制作用[J]. 国外医学中医中药分册, 2001, 23(3): 156.
[19]   Natanson C, Hoffman WD, Suffredini AF, et al. Selected treatment strategies for septic shock based on proposed mechanisms of pathogenesis[J]. Ann Intern Med, 1994, 120(9): 771-783.
[20]   Noda T, Amano F. Differences in nitric oxide synthase activity in a macrophage-like cell line, RAW264.7 cells, treated with lipopolysaccharide (LPS) in the presence or absence of interferon-gamma (IFN-gamma): possible heterogeneity of iNOS activity[J]. J Biochem, 1997, 121(1): 38-46.
[21]   霍汝亚寒. 急性肾炎患儿治疗前后血清hs-CRP、lL-2、lL-6和GM-CSF检测的临床意义[J]. 放射免疫学杂志, 2010, 23(2): 133-134.
[22]   Aldieri E, Atragene D, Bergandi L, et al. Artemisinin inhibits inducible nitric oxide synthase and nuclear factor NF-κB activation[J]. FEBS Lett, 2003, 552(2-3): 141-144.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133