%0 Journal Article
%T 小鼠肾炎模型的制备及双氢青蒿素对其炎症因子释放的影响
%A 吴苹
%A 李启杰
%A 夏？亮
%A 张发强
%A 夏庆杰
%J 华西医学
%P 1028-1031
%D 2011
%X 【】　目的　制备小鼠肾炎模型并观察双氢青蒿素（dihydroartemisinine，DHA）对模型小鼠细胞因子肿瘤坏死因子-α（tunornecrosisfactor，TNF-α）和白细胞介素-6（interleukin-6，IL-6）的影响以及小鼠肾脏的病理变化。　方法　取雄性昆明种小鼠120只，随机分为正常对照组、脂多糖（lipopolysaccharides，LPS）组、LPS+肾匀浆组及DHA治疗组；分别于12、24、48h取血，酶联免疫吸附试验检测血清中TNF-α和IL-6的含量，苏木精-伊红染色法观察小鼠肾脏的病理变化。　结果　造模48hLPS+肾匀浆组小鼠肾小球出现炎性细胞浸润，而正常对照组未见异常；LPS组及DHA治疗组仅有轻微的病理改变。LPS刺激使小鼠血清TNF-α和IL-6含量高于正常水平（P0.05）。　结论　运用改良的造模方法LPS+肾匀浆建立肾炎模型效果良好；DHA可以调节模型小鼠炎症因子TNF-α和IL-6的释放，具有一定的改善模型小鼠肾炎症状的作用。【Abstract】　Objective　Toestablishmicenephritismodels,detecttheserumlevelchangesofcytokinestumornecrosisfactorα(TNF-α)andinterleukin-6(IL-6)inthemodelmicetreatedbyDihydroartemisinin(DHA),andobservethephysiologicalchangesofthemicekidneys.　Methods　OnehundredandtwentymaleKunmingmicewererandomlydividedinto4groupscontrolgroup,lipopolysaccharides(LPS)group,LPSpluskidneyhomogenategroup,andDHAtreatedgroup.ThelevelofcytokinesTNF-αandIL-6intheserumweredetectedbyenzyme-linkedimmunoabsordentasseyathour12,24,and48,respectively.Pathologicalchangeswereobservedbyhematoxylineosinstaining.　Results　Atthetimehour48aftertheestablishmentofthemodel,inflammatorycellinfiltrationwasobservedintheglomerulusoftheLPSpluskidneyhomogenategroup,butnoabnormalitywasfoundinthecontrolgroup.TherewereonlyslightpathologicalchangesinmicemodelsoftheLPSgroupandtheDHAtreatedgroup.TheserumlevelofTNF-αandIL-6increasedremarkablyafterthetreatmentofLPS(P0.05).　Conclusion　ThemodifiedLPSpluskidneyhomogenatehasagoodresultinmodelestablishing.DHAcanregulatethereleaseofTNF-αandIL-6inthemodelmice,andmayhavecertaingoodeffectsonamelioratingthenephritispathologicalchanges.
%K 肾炎模型
%K 双氢青蒿素
%K 脂多糖
%K 肿瘤坏死因子-α
%K 白细胞介素-6
%K 小鼠
%U http://www.hxyxqk.com.cn/oa/DArticle.aspx?type=view&id=201107021