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华西医学  2011 

佛波酯诱导的蛋白激酶C活化对扭转蛋白A亚细胞分布的影响

, PP. 321-324

Keywords: 佛波酯,蛋白激酶C,扭转蛋白A,肌张力障碍

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Abstract:

【】 目的 探讨佛波酯激活的蛋白激酶C与扭转蛋白A在亚细胞成分中的表达之间的关系。 方法 采用免疫荧光法观察扭转蛋白A在原代培养的神经元和小鼠胚胎成纤维细胞(NIH3T3细胞)中的分布。运用蛋白质印迹法分析蛋白激酶C和扭转蛋白A在细亚细胞成分中的表达。 结果 扭转蛋白A在NIH3T3细胞中的表达类似于神经元。扭转蛋白A在细胞质溶质、膜成分中均有分布。佛波酯活化蛋白激酶C后并不引起扭转蛋白A在细胞质成分和膜成分中表达含量的变化。 结论 扭转蛋白A可能是膜相关蛋白,细胞氧化应激中扭转蛋白A表达上调和重分布变化不是由佛波酯诱导的蛋白激酶C活化途径来实现的。鉴于扭转蛋白A表达上调具有潜在的治疗原发性早发扭转性肌张力障碍的前景,影响其分布和表达的分子机制需要进一步研究。【Abstract】 Objective Toinvestigatetherelationshipbetweenthephorbol12-myristate13-acetate(PMA)activatedproteinkinaseC(PKC)andthesubcellularexpressionofTorsinAprotein. Methods TheexpressionofTorsinAintheprimaryculturedneuronsandtheNIH3T3cellswasdetectedbyimmunofluorescence.TheexpressionofPKCandTorsinAinsubcellularfractionwasanalyzedbythewesternblotting. Results TheexpressionpatternofTorsinAinNIH3T3cellswassimilartoneuron.PMA,anactivatorofPKC,didnotpromotetheup-expressionofTorsinAorredistributioninthesubcellularfractionofNIH3T3cells. Conclusions TorsinAmaybeamembrane-associatedprotein.Theup-regulationandredistributionofTorsinAisnotcausedbythepathwayofthePMAactivatingPKCaftercellsinsultedbyoxidativestress.WeshouldpaymoreattentiononthemechanismsoftheexpressionofTorsinAproteinforthepotentialtherapiestoearly-onsetprimarytorsiondystonia(DYT1).

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