OALib Journal期刊
磺甲基化木质素的辣根过氧化物酶催化聚合研究
DOI: 10.3724/SP.J.1105.2014.13299
Keywords: 辣根过氧化物酶 ,磺甲基化碱木质素 ,聚合 ,反应活性
Abstract:
以碱木质素为原料，采用磺甲基化反应，得到磺甲基化木质素（SAL），并进一步采用辣根过氧化物酶（HRP）催化以提高其分子量，制备了高分子量高磺化度磺甲基化木质素（HPSAL）.采用凝胶渗透色谱、红外光谱、核磁共振氢谱、紫外光谱和顶空气相色谱等研究了改性前后SAL的结构特征.结果表明，经HRP催化后，与SAL相比，HPSAL的重均分子量和磺化度显著增加，分别提高20倍和30%以上，羧基含量升高，而甲氧基和酚羟基含量降低.HRP使SAL形成酚氧自由基，活化其酚羟基的邻、对和侧链Cβ位，增加磺化反应活性，而磺化度的提高又有利于增加HRP催化SAL的聚合反应活性，其聚合方式主要为β-O-4’、β-β’、β-1’和β-5’联结.分子模拟结果表明，甲氧基含量的降低和磺酸基含量的增加能显著提高以β-O-4’连接键为主的聚合反应活性.
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