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微囊藻毒素(MC-LR)在黑麦草幼苗体内的积累及对其生长的影响

Keywords: 藻毒素,黑麦草,发芽率,幼苗生长,抗氧化酶,积累

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Abstract:

采用室内发芽试验方法,研究了不同浓度微囊藻毒素MC-LR对黑麦草种子发芽率、幼苗生长及幼苗体内抗氧化酶SOD、POD和CAT活性的影响,同时用液相色谱/质谱法(L/MS法)检测MC-LR在黑麦草幼苗体内的积累。结果显示,随着MC-LR处理浓度升高,黑麦草种子发芽率逐渐降低;MC-LR对黑麦草幼苗株高和干重无显著影响;但4mg·L-1MC-LR处理对幼苗根长和鲜重具有显著抑制作用。MC-LR处理提高了黑麦草幼苗体内SOD和POD活性,但高浓度MC-LR对两种酶活性又具有一定抑制作用。随着MC-LR处理浓度升高,MC-LR在幼苗体内积累含量和生物富集系数逐渐增大。黑麦草幼苗体内可积累MC-LR,这有可能通过食物链途径对食品安全造成一定潜在风险。

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