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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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烟曲霉产壳聚糖酶液体发酵条件研究
, PP. 34-37
Keywords: 烟曲霉(Aspergillus,fumigatus),壳聚糖酶,液体发酵
Abstract:
以烟曲霉(Aspergillusfumigatus)为出发菌,通过选择适合的培养条件,筛选出产壳聚糖酶的菌株。实验结果表明,烟曲霉是良好的产壳聚糖酶出发菌。烟曲霉液体发酵产壳聚糖酶最适培养基组成为:壳聚糖1%,KH2PO10.06%,尿素0.2%,(NH4)2SO40.1%,MgSO40.012%,20%土豆汁3%,NaCl0.05%。在pH5.0、温度28℃、摇床转速120r/min培养条件下振荡培养3d,烟曲霉产壳聚糖酶的能力最强,达25.1U/mL。
| [1] | Ohtakara A.Microbial chitosanases:the degradation of chitosan and their application[J].Nippon Nogeikagaku Kaishi,1988,62(8):49-52.
|
| [2] | Suzuki S.Immuopotentiating function of N-acetylchito-oligosaccharides and chito-oligosaccharides[J].Nippon Nogeikagaku Kaishi,1988,62(8):41-43.
|
| [3] | Pantaleone D,Yalpani M,Scollar M.Unusual susceptibilities of chitosan to enzymatic hydrolysis[J].Carbohydr Res,1992,237:325-332.
|
| [4] | Yalpani M,Pantaleone D.An examination of the unusual susceptibilities of aminoglycans to enzymatic hydrolysis[J].Carbohydr Res,1994,256:159-175.
|
| [5] | You J J,Kim S.Continuous production of chito-oligosaccharides using a dual reactor system[J].Process Biochem,2000,5(6):623-632.
|
| [6] | Monoghan R L,Eveleigh D E,Tewari R P.Chitosanases,a novel enzyme[J].Nature,1973,245(142):78-80.
|
| [7] | 方祥年,杜昱光,黄秀梨,球孢白僵菌高壳聚糖酶突变株的筛选[J].微生物学通报,2001,28(3):60-64.
|
| [8] | 王钦宏,蔡静平.壳聚糖酶生产菌的筛选、鉴定及其产酶培养条件的研究[J].工业微生物,2000,30(4):32-36.
|
| [9] | 葛正红,曾嘉.青霉菌产壳聚糖酶的分离纯化及性质研究[J].中国医药工业杂志,2002,33(8):378-381.
|
| [10] | 张维杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999,10-11.
|
| [11] | Uchida Y,Ohtakara A.Chitosanase from Bacillus sp.[J].Methods Enzymol,1988,161:501-505.
|
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