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海洋科学  2005 

盐藻胞浆hsp70 cDNA的克隆及其mRNA的诱导表达

, PP. 43-49

Keywords: 盐藻(Dunalilla,salina),hsp70,RACE,热休克,光诱导

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Abstract:

用cDNA末端快速扩增方法克隆得到了一个2652bp的盐藻(Dunalillasalina)胞浆hsp70cDNA全长,编码着650个氨基酸残基的多肽。推导的氨基酸序列有2个ATP酶结合位点和一个多肽结合区。由克隆得到的盐藻cDNA推导的氨基酸序列,经GenBankblast后与数据库中胞浆hsp70序列一致。与莱茵衣藻(Chlamydomonasreinhardtii)、人、小麦(Triticumaestivum)和啤酒酵母(Saccharomycescerevisiae)胞浆hsp70的序列一致性分别为83%、80%、81.5%和80.5%。Northern印迹显示,90min后,mRNA量在40℃热休克时是光诱导时的3倍。光诱导则使hsp70mRNA积累相对迟缓。结果表明,所克隆得到的hsp70基因蛋白产物定位在盐藻细胞浆中,光诱导和热休克均可以使hsp70mRNA水平明显增高,但以热休克为显著。

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